Isolation and Functional Characterization of Yeasts from Fermented Plant Based Products
Yeasts isolated from fermented plant-based sources—boza, pickles, and chickpeas—were evaluated for probiotic potential. Among 18 colonies, seven isolates showing no hemolytic activity were selected and identified: <i>Pichia kudriavzevii</i> (four isolates), <i>Kazachstania exigua&l...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/6/305 |
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| Summary: | Yeasts isolated from fermented plant-based sources—boza, pickles, and chickpeas—were evaluated for probiotic potential. Among 18 colonies, seven isolates showing no hemolytic activity were selected and identified: <i>Pichia kudriavzevii</i> (four isolates), <i>Kazachstania exigua</i>, <i>Hanseniaspora uvarum,</i> and <i>Saccharomyces cerevisiae</i>. Most isolates were able to survive under low pH and bile salt conditions, simulating the environment of the gastrointestinal tract (5.62–8.59 log CFU/mL) and tolerated NaCl concentrations up to 6–8% (<i>w</i>/<i>v</i>). All isolates exhibited antibiotic resistance; however, their susceptibility to antifungals varied. While <i>P. kudriavzevii</i> DD_B_M88 exhibited the highest hydrophobicity (63.07%), isolate auto-aggregation increased to 53–95% after 24 h. Most isolates showed a great capability to co-aggregate with pathogens and inhibited them significantly (up to 98.72%), except for <i>S. cerevisiae</i> DD_NB_M90. The selected three isolates and their cell-free supernatants, up to a certain concentration, showed no significant cytotoxicity on Caco-2 cell line. Eventually, six isolates, excluding <i>S. cerevisiae</i> DD_NB_M90, fulfilled the probiotic criteria, and can serve as probiotic starter cultures for alternative food production. |
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| ISSN: | 2311-5637 |