HPLC-MS/MS Method for Quantitation of Sildenafil and Its Active Metabolite in Human Plasma

QR Code

HPLC-MS/MS Method for Quantitation of Sildenafil and Its Active Metabolite in Human Plasma

A high demand for sildenafil-based drugs puts a premium on the development of methods for quantitation of sildenafil in bio-substrates to facilitate pharmacokinetic analysis in bioequivalence studies. High performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was propo...

Full description

Saved in:

Bibliographic Details
Main Authors:	G. G. Rodionov, I. I. Shantyr, I. E. Ushal, E. V. Svetkina, E. A. Kolobova, K. A. Zakharov
Format:	Article
Language:	Russian
Published:	Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’) 2020-09-01
Series:	Регуляторные исследования и экспертиза лекарственных средств
Subjects:	validation sildenafil n-desmethyl sildenafil bioequivalence hplc-ms/ms
Online Access:	https://www.vedomostincesmp.ru/jour/article/view/305
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Development and Validation of Pomalidomide Determination in Human Plasma by HPLC-MS/MS Method
by: T. N. Komarov, et al.
Published: (2020-11-01)

Development and Validation of Valganciclovir and its Active Metabolite Ganciclovir Determination in Human Plasma by HPLC-MS/MS Method
by: T. N. Komarov, et al.
Published: (2021-02-01)

Development and Validation of Tranexamic Acid Determination in Human Plasma by HPLC-MS/MS method
by: A. V. Aleshina, et al.
Published: (2021-05-01)

Development and Validation of Eltrombopag Determination in Human Plasma Blood by HPLC-MS Method
by: А. М. AL-Dhuraibi, et al.
Published: (2022-11-01)

Determination of Favipiravir in Human Blood Plasma by HPLC-MS/MS
by: T. N. Komarov, et al.
Published: (2023-09-01)

Development and Validation of Tadalafil Determination in Human Plasma by HPLC-MS Method
by: D. S. Bogdanova, et al.
Published: (2019-05-01)

HPLC-MS/MS method development and validation for the determination of tetradecapeptide in human plasma
by: M. A. Tokareva, et al.
Published: (2024-06-01)

EXPRESS METHOD FOR DETERMINATION OF ENALAPRIL AND ENALAPRILAT IN HUMAN SERUM BY HPLC-MS/MS
by: T. A. Rodina, et al.
Published: (2019-01-01)

DETERMINATION OF CAPECITABINE AND ITS ACTIVE METABOLITE 5-FLUOROURACIL IN BLOOD PLASMA BY HPLC-MS/MS
by: E. S. Melnikov, et al.
Published: (2019-01-01)

Simultaneous Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC-MS/MS
by: P. K. Karnakova, et al.
Published: (2021-11-01)

Development of a High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS) Method to Determine the Presence of Rivastigmine in Human Plasma in Clinical Studies of Comparative Pharmacokinetics
by: M. S. Dolov, et al.
Published: (2023-02-01)

Simultaneous Determination of Nirmatrelvir and Ritonavir in Human Plasma by HPLC-MS/MS
by: T. N. Komarov, et al.
Published: (2023-05-01)

Development and Validation of an HPLC-MS/MS Method for the Quantitative Determination of Etmaben in Human Blood Plasma
by: P. K. Karnakova, et al.
Published: (2024-03-01)

Development and Validation of an HPLC-MS/MS Method for Quantification of Apixaban in Human Plasma
by: U. D. Filonova, et al.
Published: (2024-03-01)

The experience in the development and validation of method for testosterone measurement in blood serum of premenopausal women using HPLC-MS/MS
by: L. V. Suturina, et al.
Published: (2022-12-01)

Mitoxantrone Quantification by HPLC-MS/MS in Caco-2 Culture Media
by: Yu. S. Tranova, et al.
Published: (2023-03-01)

Development and Validation of Salbutamol, Bromhexine, Ambroxol and Guauaifenesin Determination in Human Plasma by HPLC-MS/MS Method
by: T. N. Komarov, et al.
Published: (2019-11-01)

Bioequivalence study of ondansetron tablets by HPLC-MS
by: E. S. Stepanova, et al.
Published: (2014-04-01)

Comparative study of perindopril and perindopril metabolite pharmacokinetics using the HPLC/MS method
by: L. M. Krasnykh, et al.
Published: (2018-02-01)

Development and validation of a method for the quantitative determination of monoamine neurotransmitters and their metabolites in rat brain tissue using HPLC-MS/MS
by: A. L. Khokhlov, et al.
Published: (2024-03-01)

Development and Validation of Atazanavir and Ritonavir Determination in Human Plasma by HPLC-MS Method
by: T. N. Komarov, et al.
Published: (2020-02-01)

Simultaneous Determination of Major Molnupiravir Metabolite (β-D-N4-hydroxycytidine) and Favipiravir in Human Plasma by HPLC-MS/MS
by: T. N. Komarov, et al.
Published: (2023-02-01)

Development and validation of the method for the quantitative determination of methotrexate in a transport medium by HPLC-MS/MS
by: P. Yu. Mylnikov, et al.
Published: (2021-09-01)

Development and Validation of HPLC-MS/MS Method for Busereline Quantitation in Animal Blood Plasma
by: E. N. Fisher, et al.
Published: (2019-09-01)

Chromatography-tandem MASS spectrometry (HPLC-MS/MS) for the detection of valproic acid and its metabolites in blood plasma
by: A. S. Malygin, et al.
Published: (2018-08-01)

Quantitative determination of monoamine neurotransmitters in rat brain homogenates using HPLC-MS/MS
by: N. S. Popov, et al.
Published: (2023-01-01)

Pulmonary hypertension syndrome in dilation cardiomyopathy patients: sildenafil application to common treatment of chronic heart failure
by: T. A. Abdullaev, et al.
Published: (2016-02-01)

Validation of a method for the quantitative determination of narcotic and psychotropic substances in urine by UHPLC–MS/MS
by: N. B. Savelieva, et al.
Published: (2022-07-01)

Using gc-ms and hplc-ms/ms for analyzing impurities in triethylene glycol accumulating in the process of natural gas drying
by: M. N. Usachev, et al.
Published: (2015-08-01)

CORRECTION OF EXPERIMENTAL RETINAL ANGIOPATHY OF HYPERTENSIVE TYPE BY MINOXIDIL, SILDENAFIL
by: A. A. PERESYPKINA, et al.
Published: (2017-05-01)

Pharmacological test with sildenafil at pulmonary hypertension assessing in children with congenital heart diseases
by: O. S. Yanulevich, et al.
Published: (2010-10-01)

Pharmacokinetics Study of a New Isoxazole Derivative in Rats Using HPLC-MS/MS for Blood Sample Analysis
by: I. I. Yaichkov, et al.
Published: (2024-07-01)

Methodology for the quantitative determination of fenbendazole and its metabolites in organs and tissues of animals by high performance liquid chromatography with tandem mass spectrometry detection
by: A. I. Varlamova, et al.
Published: (2021-12-01)

Development and Validation of the HPLC Method for Quantification of the Innovative Drug DD217, Factor Xa Inhibitor, in Rat Plasma for a Pharmacokinetic Study
by: N. S. Dubovik, et al.
Published: (2022-05-01)

Risk of Glaucoma in Men Taking Sildenafil: A Propensity-Score-Matched Study
by: Kannan J. Freyaldenhoven, et al.
Published: (2025-04-01)

Development and validation of a high-performance liquid chromatography with tandem mass spectrometry method for quantification of tofacitinib in human plasma
by: E. S. Vetrova, et al.
Published: (2024-06-01)

Sildenafil effect on testosterone-induced prostate hypertrophy and relaxation of urinary bladder neck muscles
by: Qasim A. El-Dwairi, et al.
Published: (2025-12-01)

Quantitative determination of phenazepam and its active metabolite in human blood plasma at different extraction procedures
by: I. I. Miroshnichenko, et al.
Published: (2024-09-01)

Determination of Lappaconitin, Diterpene Alkaloide Obtained from Plants <i>Aconitum leucostomum</i>, and its Active Metabolite N-desacetyllappaconitin in Human Plasma and Blood
by: O. A. Archakova, et al.
Published: (2021-08-01)

Quantitative Content Parameter in the Standardization of Veratrum Aqua, Veratrum Lobelianum Bernh. Based Drug
by: E. V. Melnik, et al.
Published: (2021-02-01)