Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L.
This research aims to investigate the chemical profile of methanolic (MeOH) extracts of callus and somatic embryogenesis (SE) induced from date palm ‘Barhi’ cv. (Phoenix dactylifera L.) by total polyphenols estimation and gas chromatography-mass spectrometry (GC-MS) analysis. In addition, some biol...
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AcademicPres
2025-06-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/14374 |
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| author | Ammar M.A. ALI Ahmed A. QAHTAN Jameel M. AL-KHAYRI Methaq N. ALGABR Othman M. AL-DOSSARY Bader ALSUBAIE |
| author_facet | Ammar M.A. ALI Ahmed A. QAHTAN Jameel M. AL-KHAYRI Methaq N. ALGABR Othman M. AL-DOSSARY Bader ALSUBAIE |
| author_sort | Ammar M.A. ALI |
| collection | DOAJ |
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This research aims to investigate the chemical profile of methanolic (MeOH) extracts of callus and somatic embryogenesis (SE) induced from date palm ‘Barhi’ cv. (Phoenix dactylifera L.) by total polyphenols estimation and gas chromatography-mass spectrometry (GC-MS) analysis. In addition, some biological aspects as antiradical and anti-enzymes inhibitory effects were evaluated by in vitro examinations. Results demonstrated that, total phenol content (TPC) and total tannin content (TTC) were significantly increased by 14.95% and by 16.83%, respectively, in callus than that estimated in SE. Whereas total flavonoid content (TFC) in SE recorded a very high increase rate of 564.4% above that found in callus. GC-MS chromatogram of SE extract had more phytochemicals about 35 molecules were identified, including five specific bioactive compounds with considered ratios ranging between 2.265% and 12.76%. Overall, the determined biological aspects were significantly improved (p < 0.05) in SE, particularly anti-Diphenyl-2-picryl-hydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), and Total Antioxidant Capacity (TAC) by 48%, 92.31%, and 54.35% sequentially as antiradical/antioxidant potentials in terms of IC50 values. Furthermore, anti-α-amylase and anti-α-glucosidase effects were enhanced by 2,750% and 224.16%, respectively, in SE compared to callus results. In summary, MeOH extract of SE was richer in phytochemicals with greater bioactive properties than callus; this evaluated superiority could be attributed to the developmental and morphological differentiation occurring for SE tissues, which may be a prerequisite for greater content production and accumulation of many types of bioactive secondary metabolites. Further prospective pharmacological studies are required to recommend SE of ‘Barhi’ cv. as a natural nutritional and therapeutic source.
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| format | Article |
| id | doaj-art-a3ffa06e6a6c44b6a6b0f0029b5b71d2 |
| institution | Matheson Library |
| issn | 0255-965X 1842-4309 |
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| publishDate | 2025-06-01 |
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| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj-art-a3ffa06e6a6c44b6a6b0f0029b5b71d22025-07-02T20:15:47ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092025-06-0153210.15835/nbha53214374Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L.Ammar M.A. ALI0Ahmed A. QAHTAN1Jameel M. AL-KHAYRI2Methaq N. ALGABR3Othman M. AL-DOSSARY4Bader ALSUBAIE5Hajjah University, Faculty of Education, Department of Biology, Hajjah; Modern Specialized University, Faculty of Medical Sciences, Department of Pharmacy, Sana'a 72738, YemenKing Saud University, College of Science, Department of Botany and Microbiology, Riyadh 11451, Saudi ArabiaKing Faisal University, College of Agriculture and Food Sciences, Department of Agricultural Biotechnology, Al-Ahsa 31982Modern Specialized University, Faculty of Medical Sciences, Department of Pharmacy, Sana'a 72738King Faisal University, College of Agriculture and Food Sciences, Department of Agricultural Biotechnology, Al-Ahsa 31982King Faisal University, College of Agriculture and Food Sciences, Department of Agricultural Biotechnology, Al-Ahsa 31982 This research aims to investigate the chemical profile of methanolic (MeOH) extracts of callus and somatic embryogenesis (SE) induced from date palm ‘Barhi’ cv. (Phoenix dactylifera L.) by total polyphenols estimation and gas chromatography-mass spectrometry (GC-MS) analysis. In addition, some biological aspects as antiradical and anti-enzymes inhibitory effects were evaluated by in vitro examinations. Results demonstrated that, total phenol content (TPC) and total tannin content (TTC) were significantly increased by 14.95% and by 16.83%, respectively, in callus than that estimated in SE. Whereas total flavonoid content (TFC) in SE recorded a very high increase rate of 564.4% above that found in callus. GC-MS chromatogram of SE extract had more phytochemicals about 35 molecules were identified, including five specific bioactive compounds with considered ratios ranging between 2.265% and 12.76%. Overall, the determined biological aspects were significantly improved (p < 0.05) in SE, particularly anti-Diphenyl-2-picryl-hydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), and Total Antioxidant Capacity (TAC) by 48%, 92.31%, and 54.35% sequentially as antiradical/antioxidant potentials in terms of IC50 values. Furthermore, anti-α-amylase and anti-α-glucosidase effects were enhanced by 2,750% and 224.16%, respectively, in SE compared to callus results. In summary, MeOH extract of SE was richer in phytochemicals with greater bioactive properties than callus; this evaluated superiority could be attributed to the developmental and morphological differentiation occurring for SE tissues, which may be a prerequisite for greater content production and accumulation of many types of bioactive secondary metabolites. Further prospective pharmacological studies are required to recommend SE of ‘Barhi’ cv. as a natural nutritional and therapeutic source. https://www.notulaebotanicae.ro/index.php/nbha/article/view/14374chemical profiledate palmin vitro regenerationpharmaceutical propertiesplant tissue culture |
| spellingShingle | Ammar M.A. ALI Ahmed A. QAHTAN Jameel M. AL-KHAYRI Methaq N. ALGABR Othman M. AL-DOSSARY Bader ALSUBAIE Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. Notulae Botanicae Horti Agrobotanici Cluj-Napoca chemical profile date palm in vitro regeneration pharmaceutical properties plant tissue culture |
| title | Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. |
| title_full | Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. |
| title_fullStr | Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. |
| title_full_unstemmed | Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. |
| title_short | Enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of Phoenix dactylifera L. |
| title_sort | enhancing phytochemical content and bioactive aspects in somatic embryogenesis developed from callus of phoenix dactylifera l |
| topic | chemical profile date palm in vitro regeneration pharmaceutical properties plant tissue culture |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/14374 |
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