APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL
The article presents the results of the first stage of development of a new quantitative method for human chorionic gonadotropin (hCG) determination by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) to uncover doping abuse by athletes. The iden...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2017-02-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/75 |
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| author | I. O. Zvereva N. B. Savelieva P. V. Postnikov Yu. A. Efimova M. A. Dikunets |
| author_facet | I. O. Zvereva N. B. Savelieva P. V. Postnikov Yu. A. Efimova M. A. Dikunets |
| author_sort | I. O. Zvereva |
| collection | DOAJ |
| description | The article presents the results of the first stage of development of a new quantitative method for human chorionic gonadotropin (hCG) determination by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) to uncover doping abuse by athletes. The identified tryptic peptides correspond to the most abundant hCG isoforms: the α- and β-subunits, the nicked and β-core fragment of the hormone. Identification and sequencing of specific fragments were performed with the use of nanoLC-MS/MS. A high resolution / high accuracy hybrid mass-spectrometer was applied. Optimization of mass-spectrometric determination of selected specific peptides was accomplished by UPLC-MS/MS. Quantitative evaluation of hCG using specific fragments determination by UPLC-MS/MS allows to detect corresponding hCG isoforms. This significantly increases the method specificity and decreases the probability of false-positive results. |
| format | Article |
| id | doaj-art-a3b7dd5eb31d40dc8a88dca10ec678c6 |
| institution | Matheson Library |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| publishDate | 2017-02-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj-art-a3b7dd5eb31d40dc8a88dca10ec678c62025-08-04T10:19:38ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752017-02-01121647510.32362/2410-6593-2017-12-1-64-7575APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROLI. O. Zvereva0N. B. Savelieva1P. V. Postnikov2Yu. A. Efimova3M. A. Dikunets4Federal State Budgetary Institution “Antidoping Centre”Federal State Budgetary Institution “Antidoping Centre”Federal State Budgetary Institution “Antidoping Centre”Moscow Technological University (Institute of Fine Chemical Technologies)Federal State Budgetary Institution “Antidoping Centre”The article presents the results of the first stage of development of a new quantitative method for human chorionic gonadotropin (hCG) determination by means of ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) to uncover doping abuse by athletes. The identified tryptic peptides correspond to the most abundant hCG isoforms: the α- and β-subunits, the nicked and β-core fragment of the hormone. Identification and sequencing of specific fragments were performed with the use of nanoLC-MS/MS. A high resolution / high accuracy hybrid mass-spectrometer was applied. Optimization of mass-spectrometric determination of selected specific peptides was accomplished by UPLC-MS/MS. Quantitative evaluation of hCG using specific fragments determination by UPLC-MS/MS allows to detect corresponding hCG isoforms. This significantly increases the method specificity and decreases the probability of false-positive results.https://www.finechem-mirea.ru/jour/article/view/75human chorionic gonadotropinhcgisoformstrypsinolysisultra-hplcanti-doping controlmassspectrometry |
| spellingShingle | I. O. Zvereva N. B. Savelieva P. V. Postnikov Yu. A. Efimova M. A. Dikunets APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL Тонкие химические технологии human chorionic gonadotropin hcg isoforms trypsinolysis ultra-hplc anti-doping control massspectrometry |
| title | APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL |
| title_full | APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL |
| title_fullStr | APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL |
| title_full_unstemmed | APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL |
| title_short | APPROACHES TO CHORIONIC GONADOTROPIN QUANTITATIVE DETERMINATION IN ANTI-DOPING CONTROL |
| title_sort | approaches to chorionic gonadotropin quantitative determination in anti doping control |
| topic | human chorionic gonadotropin hcg isoforms trypsinolysis ultra-hplc anti-doping control massspectrometry |
| url | https://www.finechem-mirea.ru/jour/article/view/75 |
| work_keys_str_mv | AT iozvereva approachestochorionicgonadotropinquantitativedeterminationinantidopingcontrol AT nbsavelieva approachestochorionicgonadotropinquantitativedeterminationinantidopingcontrol AT pvpostnikov approachestochorionicgonadotropinquantitativedeterminationinantidopingcontrol AT yuaefimova approachestochorionicgonadotropinquantitativedeterminationinantidopingcontrol AT madikunets approachestochorionicgonadotropinquantitativedeterminationinantidopingcontrol |