Antimicrobial Potential of Bacteriophages JG005 and JG024 Against <i>Pseudomonas aeruginosa</i> Isolates from Canine Otitis
Canine otitis externa caused by <i>Pseudomonas aeruginosa</i> is a relevant disease in veterinary medicine. Given <i>P. aeruginosa’s</i> high priority status for the development of new antimicrobials, innovative strategies like bacteriophage therapy are essential. Lytic bacte...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/12/7/646 |
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| Summary: | Canine otitis externa caused by <i>Pseudomonas aeruginosa</i> is a relevant disease in veterinary medicine. Given <i>P. aeruginosa’s</i> high priority status for the development of new antimicrobials, innovative strategies like bacteriophage therapy are essential. Lytic bacteriophages are viruses with high specificity for their bacterial hosts, making them a promising therapeutic choice in both human and veterinary medicine. This study aimed to evaluate the antimicrobial potential of bacteriophages JG005 and JG024, first characterized in terms of their biofilm-forming ability and antimicrobial susceptibility profile, against <i>P. aeruginosa</i> isolates obtained from dogs with otitis externa,. Bacteriophages titer, host range, and activity were assessed against <i>P. aeruginosa</i> biofilms via microtiter assays using crystal violet and Alamar Blue. JG024 showed lytic activity against 61.2% (n = 30/49) of the isolates, while JG005 showed lytic activity against 38.8% (n = 19/49) of the isolates. Crystal violet quantification showed that JG005 can promote strong microbial suppression of 60% (n = 6/10) and 50% (n = 5/10) of the isolates at a multiplicity of infection (MOI) of 10 and 100, respectively. JG024 presented strong microbial suppression of 20% (n = 2/10) of the isolates regardless of the MOI level tested. These phages show promising potential as an innovative treatment for canine otitis externa caused by <i>P. aeruginosa</i>, but further studies are needed before future clinical use. |
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| ISSN: | 2306-7381 |