Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography
Aim. Carotenoids are a common and important class of natural fat-soluble pigments. The prevention of free radicals formation is the carotenoid’s primary effect. One of the tasks while creating medicines which contain carotenoids is the identification of them in plant objects. Methods and results....
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| Format: | Article |
| Language: | English |
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Zaporizhzhia State Medical and Pharmaceutical University
2015-08-01
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| Series: | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
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| Online Access: | http://pharmed.zsmu.edu.ua/article/view/45156/43753 |
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| author | E. A. Degtyaryovа V. A. Grudko L. I. Vishnevskaya E. I. Bisaga |
| author_facet | E. A. Degtyaryovа V. A. Grudko L. I. Vishnevskaya E. I. Bisaga |
| author_sort | E. A. Degtyaryovа |
| collection | DOAJ |
| description | Aim. Carotenoids are a common and important class of natural fat-soluble pigments. The prevention of free radicals formation is the carotenoid’s primary effect. One of the tasks while creating medicines which contain carotenoids is the identification of them in plant objects.
Methods and results. Our purpose is the determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography. The article presents the experimental study of solvent systems. Although, different developers which are recommended in literature have been chosen and investigated, As a result biologically active compounds of the lipophilic character have been identified, β-carotene has been identified, solvents and reagents for the identification of β-carotene have been selected.
Conclusion. This method can be included to the quality control methods to determine β-carotene in the lipophilic pumpkins extracts. |
| format | Article |
| id | doaj-art-a28e799e7dae4ad98f7c052f4b76dd5b |
| institution | Matheson Library |
| issn | 2306-8094 2409-2932 |
| language | English |
| publishDate | 2015-08-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki |
| spelling | doaj-art-a28e799e7dae4ad98f7c052f4b76dd5b2025-08-02T15:35:09ZengZaporizhzhia State Medical and Pharmaceutical UniversityAktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki2306-80942409-29322015-08-012454810.14739/2409-2932.2015.2.45156Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatographyE. A. DegtyaryovаV. A. GrudkoL. I. VishnevskayaE. I. BisagaAim. Carotenoids are a common and important class of natural fat-soluble pigments. The prevention of free radicals formation is the carotenoid’s primary effect. One of the tasks while creating medicines which contain carotenoids is the identification of them in plant objects. Methods and results. Our purpose is the determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography. The article presents the experimental study of solvent systems. Although, different developers which are recommended in literature have been chosen and investigated, As a result biologically active compounds of the lipophilic character have been identified, β-carotene has been identified, solvents and reagents for the identification of β-carotene have been selected. Conclusion. This method can be included to the quality control methods to determine β-carotene in the lipophilic pumpkins extracts.http://pharmed.zsmu.edu.ua/article/view/45156/43753CucurbitaBiologically Active ConnectionsCarotenoidsPhytosterolsThin Layer Chromatography |
| spellingShingle | E. A. Degtyaryovа V. A. Grudko L. I. Vishnevskaya E. I. Bisaga Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki Cucurbita Biologically Active Connections Carotenoids Phytosterols Thin Layer Chromatography |
| title | Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| title_full | Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| title_fullStr | Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| title_full_unstemmed | Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| title_short | Determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| title_sort | determination of biologically active compounds in lipophilic extracts of pumpkin using thin layer chromatography |
| topic | Cucurbita Biologically Active Connections Carotenoids Phytosterols Thin Layer Chromatography |
| url | http://pharmed.zsmu.edu.ua/article/view/45156/43753 |
| work_keys_str_mv | AT eadegtyaryova determinationofbiologicallyactivecompoundsinlipophilicextractsofpumpkinusingthinlayerchromatography AT vagrudko determinationofbiologicallyactivecompoundsinlipophilicextractsofpumpkinusingthinlayerchromatography AT livishnevskaya determinationofbiologicallyactivecompoundsinlipophilicextractsofpumpkinusingthinlayerchromatography AT eibisaga determinationofbiologicallyactivecompoundsinlipophilicextractsofpumpkinusingthinlayerchromatography |