Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10
Adhesin is an important pathogenic factor of Aeromonas hydrophila which can cause disease in most fish in the area of Jiangsu and Zhejiang. The strains of TPS-30 and BSK-10 were assigned to serogroup O:9 and O:97 respectively, and the genes of the major adhesin (designated as Al) of the two strains...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.004 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839607360275873792 |
|---|---|
| author | PAN Xiao-yi SHEN Jin-yu HAO Gui-jie YAO Jia-yun XU Yang |
| author_facet | PAN Xiao-yi SHEN Jin-yu HAO Gui-jie YAO Jia-yun XU Yang |
| author_sort | PAN Xiao-yi |
| collection | DOAJ |
| description | Adhesin is an important pathogenic factor of Aeromonas hydrophila which can cause disease in most fish in the area of Jiangsu and Zhejiang. The strains of TPS-30 and BSK-10 were assigned to serogroup O:9 and O:97 respectively, and the genes of the major adhesin (designated as Al) of the two strains were cloned and sequenced. The results revealed that there was a mutation region in the N-terminal domain in the Al sequences of the two strains, and the mutation area of TPS-30 was a bit shorter than that of BSK-10. The Al of TPS-30 was cloned in the vector pET28a(+) and expressed in Escherichia coli BL21(DE3). A band of 41 ku fusion protein of A l was confirmed by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The expressed protein products reached a peak after 6 h of induction and accounted for 44.0% of whole bacterial protein. Carassius Auratus Gibelio vaccinated with the fusion adhesin produced a significant immune response against A. hydrophila. The antibody titer reached the highest at the 5th week of vaccination. The relative percentage survivals (RPS) of TPS-30 and BSK-10 were 94.7% and 77.8%, respectively. Therefore, the recombinant strain BL21 (DE3) (pET28a-AHal) could be used to develop recombinant subunit vaccines against the infection of A. hydrophila. |
| format | Article |
| id | doaj-art-a23301dbc7fa49c18f1c48436fba6da6 |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2010-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a23301dbc7fa49c18f1c48436fba6da62025-08-01T05:35:11ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552010-05-013626226810.3785/j.issn.1008-9209.2010.03.00410089209Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10PAN Xiao-yiSHEN Jin-yuHAO Gui-jieYAO Jia-yunXU YangAdhesin is an important pathogenic factor of Aeromonas hydrophila which can cause disease in most fish in the area of Jiangsu and Zhejiang. The strains of TPS-30 and BSK-10 were assigned to serogroup O:9 and O:97 respectively, and the genes of the major adhesin (designated as Al) of the two strains were cloned and sequenced. The results revealed that there was a mutation region in the N-terminal domain in the Al sequences of the two strains, and the mutation area of TPS-30 was a bit shorter than that of BSK-10. The Al of TPS-30 was cloned in the vector pET28a(+) and expressed in Escherichia coli BL21(DE3). A band of 41 ku fusion protein of A l was confirmed by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The expressed protein products reached a peak after 6 h of induction and accounted for 44.0% of whole bacterial protein. Carassius Auratus Gibelio vaccinated with the fusion adhesin produced a significant immune response against A. hydrophila. The antibody titer reached the highest at the 5th week of vaccination. The relative percentage survivals (RPS) of TPS-30 and BSK-10 were 94.7% and 77.8%, respectively. Therefore, the recombinant strain BL21 (DE3) (pET28a-AHal) could be used to develop recombinant subunit vaccines against the infection of A. hydrophila.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.004<italic>Aeromonas hydrophila</italic>adhesion geneimmunogenicityprotective antigenserotype |
| spellingShingle | PAN Xiao-yi SHEN Jin-yu HAO Gui-jie YAO Jia-yun XU Yang Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 浙江大学学报. 农业与生命科学版 <italic>Aeromonas hydrophila</italic> adhesion gene immunogenicity protective antigen serotype |
| title | Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 |
| title_full | Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 |
| title_fullStr | Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 |
| title_full_unstemmed | Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 |
| title_short | Cloning, expression and sequence analysis of Al gene in Aeromonas hydrophila strains TPS-30 and BSK-10 |
| title_sort | cloning expression and sequence analysis of al gene in aeromonas hydrophila strains tps 30 and bsk 10 |
| topic | <italic>Aeromonas hydrophila</italic> adhesion gene immunogenicity protective antigen serotype |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.03.004 |
| work_keys_str_mv | AT panxiaoyi cloningexpressionandsequenceanalysisofalgeneinaeromonashydrophilastrainstps30andbsk10 AT shenjinyu cloningexpressionandsequenceanalysisofalgeneinaeromonashydrophilastrainstps30andbsk10 AT haoguijie cloningexpressionandsequenceanalysisofalgeneinaeromonashydrophilastrainstps30andbsk10 AT yaojiayun cloningexpressionandsequenceanalysisofalgeneinaeromonashydrophilastrainstps30andbsk10 AT xuyang cloningexpressionandsequenceanalysisofalgeneinaeromonashydrophilastrainstps30andbsk10 |