On technique for counting of fish pyloric appendages

New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven dep...

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Main Author: Anna E. Lapshina
Format: Article
Language:Russian
Published: Transactions of the Pacific Research Institute of Fisheries and Oceanography 2014-06-01
Series:Известия ТИНРО
Subjects:
Online Access:https://izvestiya.tinro-center.ru/jour/article/view/22
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author Anna E. Lapshina
author_facet Anna E. Lapshina
author_sort Anna E. Lapshina
collection DOAJ
description New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven depending on weight of sample and power of the oven. The technique allows to avoid work with toxic reagents, as ethanol and formalin, and to lower the costs of investigation.
format Article
id doaj-art-a22ffaa36c204b00b6f80b200a75b2fe
institution Matheson Library
issn 1606-9919
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language Russian
publishDate 2014-06-01
publisher Transactions of the Pacific Research Institute of Fisheries and Oceanography
record_format Article
series Известия ТИНРО
spelling doaj-art-a22ffaa36c204b00b6f80b200a75b2fe2025-08-03T19:27:08ZrusTransactions of the Pacific Research Institute of Fisheries and OceanographyИзвестия ТИНРО1606-99192658-55102014-06-01177229529710.26428/1606-9919-2014-177-295-29722On technique for counting of fish pyloric appendagesAnna E. Lapshina0Сахалинское бассейновое управление по рыболовству и сохранению водных биологических ресурсов (Сахалинрыбвод)New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven depending on weight of sample and power of the oven. The technique allows to avoid work with toxic reagents, as ethanol and formalin, and to lower the costs of investigation.https://izvestiya.tinro-center.ru/jour/article/view/22pyloric appendagefixationdeep-freezingmicrowave oven
spellingShingle Anna E. Lapshina
On technique for counting of fish pyloric appendages
Известия ТИНРО
pyloric appendage
fixation
deep-freezing
microwave oven
title On technique for counting of fish pyloric appendages
title_full On technique for counting of fish pyloric appendages
title_fullStr On technique for counting of fish pyloric appendages
title_full_unstemmed On technique for counting of fish pyloric appendages
title_short On technique for counting of fish pyloric appendages
title_sort on technique for counting of fish pyloric appendages
topic pyloric appendage
fixation
deep-freezing
microwave oven
url https://izvestiya.tinro-center.ru/jour/article/view/22
work_keys_str_mv AT annaelapshina ontechniqueforcountingoffishpyloricappendages