On technique for counting of fish pyloric appendages
New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven dep...
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Format: | Article |
Language: | Russian |
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Transactions of the Pacific Research Institute of Fisheries and Oceanography
2014-06-01
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Series: | Известия ТИНРО |
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Online Access: | https://izvestiya.tinro-center.ru/jour/article/view/22 |
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author | Anna E. Lapshina |
author_facet | Anna E. Lapshina |
author_sort | Anna E. Lapshina |
collection | DOAJ |
description | New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven depending on weight of sample and power of the oven. The technique allows to avoid work with toxic reagents, as ethanol and formalin, and to lower the costs of investigation. |
format | Article |
id | doaj-art-a22ffaa36c204b00b6f80b200a75b2fe |
institution | Matheson Library |
issn | 1606-9919 2658-5510 |
language | Russian |
publishDate | 2014-06-01 |
publisher | Transactions of the Pacific Research Institute of Fisheries and Oceanography |
record_format | Article |
series | Известия ТИНРО |
spelling | doaj-art-a22ffaa36c204b00b6f80b200a75b2fe2025-08-03T19:27:08ZrusTransactions of the Pacific Research Institute of Fisheries and OceanographyИзвестия ТИНРО1606-99192658-55102014-06-01177229529710.26428/1606-9919-2014-177-295-29722On technique for counting of fish pyloric appendagesAnna E. Lapshina0Сахалинское бассейновое управление по рыболовству и сохранению водных биологических ресурсов (Сахалинрыбвод)New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven depending on weight of sample and power of the oven. The technique allows to avoid work with toxic reagents, as ethanol and formalin, and to lower the costs of investigation.https://izvestiya.tinro-center.ru/jour/article/view/22pyloric appendagefixationdeep-freezingmicrowave oven |
spellingShingle | Anna E. Lapshina On technique for counting of fish pyloric appendages Известия ТИНРО pyloric appendage fixation deep-freezing microwave oven |
title | On technique for counting of fish pyloric appendages |
title_full | On technique for counting of fish pyloric appendages |
title_fullStr | On technique for counting of fish pyloric appendages |
title_full_unstemmed | On technique for counting of fish pyloric appendages |
title_short | On technique for counting of fish pyloric appendages |
title_sort | on technique for counting of fish pyloric appendages |
topic | pyloric appendage fixation deep-freezing microwave oven |
url | https://izvestiya.tinro-center.ru/jour/article/view/22 |
work_keys_str_mv | AT annaelapshina ontechniqueforcountingoffishpyloricappendages |