MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI)
Lately some innovative selection methods like a marker-assisted selection (MAS) are used together with traditional ones that enable to develop new varieties with qualitative traits faster. MAS include conducting of PCR and analysis of received data to determine the presence of genes alleles with def...
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
2018-05-01
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| Series: | Зерновое хозяйство России |
| Subjects: | |
| Online Access: | https://www.zhros.online/jour/article/view/287 |
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| author | G. V. Vildanova V. I. Blokhin I. S. Ganieva M. M. Lanochkina |
| author_facet | G. V. Vildanova V. I. Blokhin I. S. Ganieva M. M. Lanochkina |
| author_sort | G. V. Vildanova |
| collection | DOAJ |
| description | Lately some innovative selection methods like a marker-assisted selection (MAS) are used together with traditional ones that enable to develop new varieties with qualitative traits faster. MAS include conducting of PCR and analysis of received data to determine the presence of genes alleles with definite traits or DNA-genotyping. The article gives the results of DNA-genotyping of variety samples of TatRIA selected with four markers of resistance to covered smut (aHor2, UhR450, OPO6780 and OPJ10450). The hybrids F3-F5 were the subjects of study, which were examined by PCR with the use of DNA of selected variety samples. The analysis showed that a genome of one variety sample could possess several gene alleles of resistance to covered smut. The testing of the sample with PCR-method on locus aHor2 presence showed that less than a half of them turned to be homozygous in susceptibility to covered smut. Thus the analysis helped us to determine economic valuable genotypes for their further use in breeding. Conducted field trials confirmed the received data on genotyping on resistance to covered smut. |
| format | Article |
| id | doaj-art-a1a66bd0523f4fea9a9f30bdb3f841e3 |
| institution | Matheson Library |
| issn | 2079-8725 2079-8733 |
| language | Russian |
| publishDate | 2018-05-01 |
| publisher | Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" |
| record_format | Article |
| series | Зерновое хозяйство России |
| spelling | doaj-art-a1a66bd0523f4fea9a9f30bdb3f841e32025-08-03T19:53:55ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332018-05-01053033286MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI)G. V. Vildanova0V. I. Blokhin1I. S. Ganieva2M. M. Lanochkina3FSBSI Tatar Research Institute of AgricultureFSBSI Tatar Research Institute of AgricultureFSBSI Tatar Research Institute of AgricultureFSBSI Tatar Research Institute of AgricultureLately some innovative selection methods like a marker-assisted selection (MAS) are used together with traditional ones that enable to develop new varieties with qualitative traits faster. MAS include conducting of PCR and analysis of received data to determine the presence of genes alleles with definite traits or DNA-genotyping. The article gives the results of DNA-genotyping of variety samples of TatRIA selected with four markers of resistance to covered smut (aHor2, UhR450, OPO6780 and OPJ10450). The hybrids F3-F5 were the subjects of study, which were examined by PCR with the use of DNA of selected variety samples. The analysis showed that a genome of one variety sample could possess several gene alleles of resistance to covered smut. The testing of the sample with PCR-method on locus aHor2 presence showed that less than a half of them turned to be homozygous in susceptibility to covered smut. Thus the analysis helped us to determine economic valuable genotypes for their further use in breeding. Conducted field trials confirmed the received data on genotyping on resistance to covered smut.https://www.zhros.online/jour/article/view/287spring barleydna-genotypingpcrprimermarkerallelegeneselectionresistancecovered smut |
| spellingShingle | G. V. Vildanova V. I. Blokhin I. S. Ganieva M. M. Lanochkina MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) Зерновое хозяйство России spring barley dna-genotyping pcr primer marker allele gene selection resistance covered smut |
| title | MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) |
| title_full | MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) |
| title_fullStr | MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) |
| title_full_unstemmed | MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) |
| title_short | MOLECULAR GENETIC ASSESSMENT OF BARLEY SELECTION MATERIAL ON ENDURANCE OF COVERED SMUT (USTILAGO HORDEI) |
| title_sort | molecular genetic assessment of barley selection material on endurance of covered smut ustilago hordei |
| topic | spring barley dna-genotyping pcr primer marker allele gene selection resistance covered smut |
| url | https://www.zhros.online/jour/article/view/287 |
| work_keys_str_mv | AT gvvildanova moleculargeneticassessmentofbarleyselectionmaterialonenduranceofcoveredsmutustilagohordei AT viblokhin moleculargeneticassessmentofbarleyselectionmaterialonenduranceofcoveredsmutustilagohordei AT isganieva moleculargeneticassessmentofbarleyselectionmaterialonenduranceofcoveredsmutustilagohordei AT mmlanochkina moleculargeneticassessmentofbarleyselectionmaterialonenduranceofcoveredsmutustilagohordei |