Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs)
Delayed or non-healing of bone defects in an aging, multi-morbid population is still a medical challenge. Current replacement materials, like autografts, are limited. Thus, artificial substitutes from biodegradable polymers and bioactive glasses (BGs) are promising alternatives. Here, novel cerium-d...
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MDPI AG
2025-06-01
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| author | Andrey Iodchik Gigliola Lusvardi Alfonso Zambon Poh Soo Lee Hans-Peter Wiesmann Anne Bernhardt Vera Hintze |
| author_facet | Andrey Iodchik Gigliola Lusvardi Alfonso Zambon Poh Soo Lee Hans-Peter Wiesmann Anne Bernhardt Vera Hintze |
| author_sort | Andrey Iodchik |
| collection | DOAJ |
| description | Delayed or non-healing of bone defects in an aging, multi-morbid population is still a medical challenge. Current replacement materials, like autografts, are limited. Thus, artificial substitutes from biodegradable polymers and bioactive glasses (BGs) are promising alternatives. Here, novel cerium-doped mesoporous BG microparticles (Ce-MBGs) with different cerium content were included in photocrosslinkable, methacrylated gelatin (GelMA) for promoting cellular functions of human mesenchymal stem cells (hBMSCs). The composites were studied for intrinsic morphology and Ce-MBGs distribution by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). They were gravimetrically analyzed for swelling and stability, compressive modulus via Microsquisher<sup>®</sup> and bioactivity by Fluitest<sup>®</sup> calcium assay and inductively coupled plasma-optical emission spectrometry (ICP-OES), also determining silicon and cerium ion release. Finally, seeding, proliferation, and differentiation of hBMSCs was investigated. Ce-MBGs were evenly distributed within composites. The latter displayed a concentration-dependent but cerium-independent decrease in swelling, while mechanical properties were comparable. A MBG type-dependent bioactivity was shown, while an enhanced osteogenic differentiation of hBMSCs was achieved for Ce-MBG-composites and related to different ion release profiles. These findings show their strong potential in promoting bone regeneration. Still, future work is required, e.g., analyzing the expression of osteogenic genes, providing further evidence for the composites’ osteogenic effect. |
| format | Article |
| id | doaj-art-a03a87ae2e6f47efb33e6c9746772b05 |
| institution | Matheson Library |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-a03a87ae2e6f47efb33e6c9746772b052025-06-25T13:53:49ZengMDPI AGGels2310-28612025-06-0111642510.3390/gels11060425Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs)Andrey Iodchik0Gigliola Lusvardi1Alfonso Zambon2Poh Soo Lee3Hans-Peter Wiesmann4Anne Bernhardt5Vera Hintze6Institute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden University of Technology, Budapester Str. 27, D-01069 Dresden, GermanyDepartment of Chemical and Geological Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyDepartment of Chemical and Geological Sciences, University of Modena and Reggio Emilia, 41125 Modena, ItalyInstitute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden University of Technology, Budapester Str. 27, D-01069 Dresden, GermanyInstitute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden University of Technology, Budapester Str. 27, D-01069 Dresden, GermanyCentre for Translational Bone, Joint and Soft Tissue Research, University Hospital “Carl Gustav Carus”, TU Dresden University of Technology, Fetscherstraße 74, D-01307 Dresden, GermanyInstitute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden University of Technology, Budapester Str. 27, D-01069 Dresden, GermanyDelayed or non-healing of bone defects in an aging, multi-morbid population is still a medical challenge. Current replacement materials, like autografts, are limited. Thus, artificial substitutes from biodegradable polymers and bioactive glasses (BGs) are promising alternatives. Here, novel cerium-doped mesoporous BG microparticles (Ce-MBGs) with different cerium content were included in photocrosslinkable, methacrylated gelatin (GelMA) for promoting cellular functions of human mesenchymal stem cells (hBMSCs). The composites were studied for intrinsic morphology and Ce-MBGs distribution by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). They were gravimetrically analyzed for swelling and stability, compressive modulus via Microsquisher<sup>®</sup> and bioactivity by Fluitest<sup>®</sup> calcium assay and inductively coupled plasma-optical emission spectrometry (ICP-OES), also determining silicon and cerium ion release. Finally, seeding, proliferation, and differentiation of hBMSCs was investigated. Ce-MBGs were evenly distributed within composites. The latter displayed a concentration-dependent but cerium-independent decrease in swelling, while mechanical properties were comparable. A MBG type-dependent bioactivity was shown, while an enhanced osteogenic differentiation of hBMSCs was achieved for Ce-MBG-composites and related to different ion release profiles. These findings show their strong potential in promoting bone regeneration. Still, future work is required, e.g., analyzing the expression of osteogenic genes, providing further evidence for the composites’ osteogenic effect.https://www.mdpi.com/2310-2861/11/6/425hydrogelsmethacrylated gelatincerium-doped mesoporous bioactive glasseshuman mesenchymal stem cellsosteogenic differentiation |
| spellingShingle | Andrey Iodchik Gigliola Lusvardi Alfonso Zambon Poh Soo Lee Hans-Peter Wiesmann Anne Bernhardt Vera Hintze Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) Gels hydrogels methacrylated gelatin cerium-doped mesoporous bioactive glasses human mesenchymal stem cells osteogenic differentiation |
| title | Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) |
| title_full | Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) |
| title_fullStr | Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) |
| title_full_unstemmed | Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) |
| title_short | Gelatin/Cerium-Doped Bioactive Glass Composites for Enhancing Cellular Functions of Human Mesenchymal Stem Cells (hBMSCs) |
| title_sort | gelatin cerium doped bioactive glass composites for enhancing cellular functions of human mesenchymal stem cells hbmscs |
| topic | hydrogels methacrylated gelatin cerium-doped mesoporous bioactive glasses human mesenchymal stem cells osteogenic differentiation |
| url | https://www.mdpi.com/2310-2861/11/6/425 |
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