In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect
Ratih Rinendyaputri,1,2 Ita Margaretha Nainggolan,3 Hasta Handayani Idrus,1 Rachmawati Noverina,4 Wireni Ayuningtyas,4 Fathul Huda,5 Ahmad Faried6 1Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Bogor, West Java, Indonesia; 2Doctoral Progra...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-05-01
|
| Series: | Stem Cells and Cloning: Advances and Applications |
| Subjects: | |
| Online Access: | https://www.dovepress.com/in-vitro-and-in-vivo-studies-on-mesenchymal-stem-cells-for-ischemic-st-peer-reviewed-fulltext-article-SCCAA |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839651881243115520 |
|---|---|
| author | Rinendyaputri R Nainggolan IM Idrus HH Noverina R Ayuningtyas W Huda F Faried A |
| author_facet | Rinendyaputri R Nainggolan IM Idrus HH Noverina R Ayuningtyas W Huda F Faried A |
| author_sort | Rinendyaputri R |
| collection | DOAJ |
| description | Ratih Rinendyaputri,1,2 Ita Margaretha Nainggolan,3 Hasta Handayani Idrus,1 Rachmawati Noverina,4 Wireni Ayuningtyas,4 Fathul Huda,5 Ahmad Faried6 1Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Bogor, West Java, Indonesia; 2Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, 40161, Indonesia; 3Eijkman Research Center for Molecular Biology, Research Organization for Health, National Research and Innovation Agency, Bogor, West Java, Indonesia; 4Bio Farma Stem Cell Research and Development, Bandung, West Java, 40161, Indonesia; 5Department of Neurology, Faculty of Medicine, Dr Hasan Sadikin Central General Hospital/Universitas Padjadjaran, Bandung, West Java, 40161, Indonesia; 6Neurosurgery Department, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, 40161, IndonesiaCorrespondence: Ahmad Faried, Email ahmad.faried@unpad.ac.idIntroduction: Mesenchymal stem cells (MSCs) have a paracrine impact and may regenerate a variety of tissues. This represents a new prospect in cell-based stroke treatment. Several in vitro and in vivo investigations have demonstrated the neuroprotective and neurogenesis properties of MSCs and their secretome.Purpose: This review provides a comprehensive analysis of the therapeutic effects of MSCs and their secretome on stroke models in vitro and in vivo.Methods: A coverage evaluation is undertaken in accordance with PRISMA-ScR principles. The selection procedure includes the identification of items. Scopus site, PubMed and ScienceDirect, are used for in vitro and in vitro research, including electronic searches. The search terms include “ischemic stroke” or “MCAO”, “MSC”, “secretome”, and “neurogenesis” or “angiogenesis”. The searches are limited to English-language articles with full text availability.Results: After selecting 390 papers from two search engines, 94 publications satisfied the review criteria for using MSCs and secretomes for ischemic stroke treatment. We comprehensively review both in vitro and in vivo studies, analyzing aspects such as the source and treatment of MSCs and secretomes, as well as administration, dosage, and mechanisms of therapeutic effects in stroke models.Conclusion: MSC and secretome therapy for stroke have shown promising results in both in vitro and in vivo models. Exploration of alternative MSC sources, refining of isolation techniques, transfection of various proteins, and combination with herbal medicine are all efforts to improve the preclinical model. This work can be used as a reference for preclinical researchers to help with research design and translational research in clinical trials.Keywords: middle cerebral artery occlusion (MCAO), conditioned medium/CM, secretome, mesenchymal stem cells (MSC), stroke ischemic |
| format | Article |
| id | doaj-art-9eba33c0149d49bb9aba3ae51b07ea16 |
| institution | Matheson Library |
| issn | 1178-6957 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Stem Cells and Cloning: Advances and Applications |
| spelling | doaj-art-9eba33c0149d49bb9aba3ae51b07ea162025-06-25T21:33:51ZengDove Medical PressStem Cells and Cloning: Advances and Applications1178-69572025-05-01Volume 18Issue 14561103459In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic EffectRinendyaputri R0Nainggolan IM1Idrus HH2Noverina R3Ayuningtyas W4Huda F5Faried A6Center for Biomedical Research, Research Organization for HealthEijkman Research Center for Molecular Biology, Research Organization for HealthCenter for Biomedical Research, Research Organization for HealthBio Farma Stem Cell Research and DevelopmentResearch and DevelopmentBiomedical Science, Faculty of MedicineNeurosurgery Department, Faculty of MedicineRatih Rinendyaputri,1,2 Ita Margaretha Nainggolan,3 Hasta Handayani Idrus,1 Rachmawati Noverina,4 Wireni Ayuningtyas,4 Fathul Huda,5 Ahmad Faried6 1Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency, Bogor, West Java, Indonesia; 2Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, 40161, Indonesia; 3Eijkman Research Center for Molecular Biology, Research Organization for Health, National Research and Innovation Agency, Bogor, West Java, Indonesia; 4Bio Farma Stem Cell Research and Development, Bandung, West Java, 40161, Indonesia; 5Department of Neurology, Faculty of Medicine, Dr Hasan Sadikin Central General Hospital/Universitas Padjadjaran, Bandung, West Java, 40161, Indonesia; 6Neurosurgery Department, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, 40161, IndonesiaCorrespondence: Ahmad Faried, Email ahmad.faried@unpad.ac.idIntroduction: Mesenchymal stem cells (MSCs) have a paracrine impact and may regenerate a variety of tissues. This represents a new prospect in cell-based stroke treatment. Several in vitro and in vivo investigations have demonstrated the neuroprotective and neurogenesis properties of MSCs and their secretome.Purpose: This review provides a comprehensive analysis of the therapeutic effects of MSCs and their secretome on stroke models in vitro and in vivo.Methods: A coverage evaluation is undertaken in accordance with PRISMA-ScR principles. The selection procedure includes the identification of items. Scopus site, PubMed and ScienceDirect, are used for in vitro and in vitro research, including electronic searches. The search terms include “ischemic stroke” or “MCAO”, “MSC”, “secretome”, and “neurogenesis” or “angiogenesis”. The searches are limited to English-language articles with full text availability.Results: After selecting 390 papers from two search engines, 94 publications satisfied the review criteria for using MSCs and secretomes for ischemic stroke treatment. We comprehensively review both in vitro and in vivo studies, analyzing aspects such as the source and treatment of MSCs and secretomes, as well as administration, dosage, and mechanisms of therapeutic effects in stroke models.Conclusion: MSC and secretome therapy for stroke have shown promising results in both in vitro and in vivo models. Exploration of alternative MSC sources, refining of isolation techniques, transfection of various proteins, and combination with herbal medicine are all efforts to improve the preclinical model. This work can be used as a reference for preclinical researchers to help with research design and translational research in clinical trials.Keywords: middle cerebral artery occlusion (MCAO), conditioned medium/CM, secretome, mesenchymal stem cells (MSC), stroke ischemichttps://www.dovepress.com/in-vitro-and-in-vivo-studies-on-mesenchymal-stem-cells-for-ischemic-st-peer-reviewed-fulltext-article-SCCAAmiddle cerebral artery occlusion (MCAO)conditioned medium/CMsecretomemesenchymal stem cells (MSC)stroke ischemic |
| spellingShingle | Rinendyaputri R Nainggolan IM Idrus HH Noverina R Ayuningtyas W Huda F Faried A In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect Stem Cells and Cloning: Advances and Applications middle cerebral artery occlusion (MCAO) conditioned medium/CM secretome mesenchymal stem cells (MSC) stroke ischemic |
| title | In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect |
| title_full | In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect |
| title_fullStr | In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect |
| title_full_unstemmed | In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect |
| title_short | In vitro and In vivo Studies on Mesenchymal Stem Cells for Ischemic Stroke Therapy: A Scoping Review of The Therapeutic Effect |
| title_sort | in vitro and in vivo studies on mesenchymal stem cells for ischemic stroke therapy a scoping review of the therapeutic effect |
| topic | middle cerebral artery occlusion (MCAO) conditioned medium/CM secretome mesenchymal stem cells (MSC) stroke ischemic |
| url | https://www.dovepress.com/in-vitro-and-in-vivo-studies-on-mesenchymal-stem-cells-for-ischemic-st-peer-reviewed-fulltext-article-SCCAA |
| work_keys_str_mv | AT rinendyaputrir invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT nainggolanim invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT idrushh invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT noverinar invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT ayuningtyasw invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT hudaf invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect AT farieda invitroandinvivostudiesonmesenchymalstemcellsforischemicstroketherapyascopingreviewofthetherapeuticeffect |