Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins
Zearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often time-consuming, require specialized infrastructure,...
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| Format: | Article |
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MDPI AG
2025-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/6/361 |
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| author | Kundan Kumar Mishra Krupa M. Thakkar Vikram Narayanan Dhamu Sriram Muthukumar Shalini Prasad |
| author_facet | Kundan Kumar Mishra Krupa M. Thakkar Vikram Narayanan Dhamu Sriram Muthukumar Shalini Prasad |
| author_sort | Kundan Kumar Mishra |
| collection | DOAJ |
| description | Zearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often time-consuming, require specialized infrastructure, and lack portability, limiting their utility for rapid, on-site screening. In response, this study introduces a compact, real-time electrochemical sensing platform for the swift and selective detection of ZEA in corn flour matrices. Utilizing a non-faradaic, label-free approach based on Electrochemical Impedance Spectroscopy (EIS), the sensor leverages ZEA-specific antibodies to achieve rapid detection within 5 min. The platform demonstrates a low detection limit of 0.05 ng/mL, with a broad dynamic range from 0.1 ng/mL to 25.6 ng/mL. Reproducibility tests confirm consistent performance, with both inter- and intra-assay variation remaining under a 20% coefficient of variation (%CV). Comparative evaluation with standard benchtop systems underscores its accuracy and field applicability. This portable and user-friendly device provides a powerful tool for real-time mycotoxin monitoring, offering significant potential for improving food safety practices and enabling point-of-need testing in resource-limited settings. |
| format | Article |
| id | doaj-art-9e7f74a6945348e48a7a7dd25e6ea4a2 |
| institution | Matheson Library |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-9e7f74a6945348e48a7a7dd25e6ea4a22025-06-25T13:34:02ZengMDPI AGBiosensors2079-63742025-06-0115636110.3390/bios15060361Electrochemical Sensor Platform for Rapid Detection of Foodborne ToxinsKundan Kumar Mishra0Krupa M. Thakkar1Vikram Narayanan Dhamu2Sriram Muthukumar3Shalini Prasad4Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USADepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USAEnLiSense LLC, 1813 Audubon Pondway, Allen, TX 75013, USAEnLiSense LLC, 1813 Audubon Pondway, Allen, TX 75013, USADepartment of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USAZearalenone (ZEA), a potent mycotoxin commonly found in contaminated grains, presents a serious threat to food safety and public health. Conventional detection methods, including culture-based assays and laboratory-bound analytical tools, are often time-consuming, require specialized infrastructure, and lack portability, limiting their utility for rapid, on-site screening. In response, this study introduces a compact, real-time electrochemical sensing platform for the swift and selective detection of ZEA in corn flour matrices. Utilizing a non-faradaic, label-free approach based on Electrochemical Impedance Spectroscopy (EIS), the sensor leverages ZEA-specific antibodies to achieve rapid detection within 5 min. The platform demonstrates a low detection limit of 0.05 ng/mL, with a broad dynamic range from 0.1 ng/mL to 25.6 ng/mL. Reproducibility tests confirm consistent performance, with both inter- and intra-assay variation remaining under a 20% coefficient of variation (%CV). Comparative evaluation with standard benchtop systems underscores its accuracy and field applicability. This portable and user-friendly device provides a powerful tool for real-time mycotoxin monitoring, offering significant potential for improving food safety practices and enabling point-of-need testing in resource-limited settings.https://www.mdpi.com/2079-6374/15/6/361electrochemical impedance spectroscopytoxinszearalenoneimmunosensor |
| spellingShingle | Kundan Kumar Mishra Krupa M. Thakkar Vikram Narayanan Dhamu Sriram Muthukumar Shalini Prasad Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins Biosensors electrochemical impedance spectroscopy toxins zearalenone immunosensor |
| title | Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins |
| title_full | Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins |
| title_fullStr | Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins |
| title_full_unstemmed | Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins |
| title_short | Electrochemical Sensor Platform for Rapid Detection of Foodborne Toxins |
| title_sort | electrochemical sensor platform for rapid detection of foodborne toxins |
| topic | electrochemical impedance spectroscopy toxins zearalenone immunosensor |
| url | https://www.mdpi.com/2079-6374/15/6/361 |
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