The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study
ObjectivesTo investigate the gut microbiota in children with varying degrees of idiopathic short stature (ISS) and to examine the relationship between their intestinal microbiota and lifestyle factors, including diet, exercise, medication, sleep, and psychological state.MethodsA retrospective study...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1566722/full |
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| author | Qin Zeng Qin Zeng Jun Zhu Jun Zhu Yanling Hu Yanling Hu Shaoyu Su Shaoyu Su Jing Chen Jing Chen |
| author_facet | Qin Zeng Qin Zeng Jun Zhu Jun Zhu Yanling Hu Yanling Hu Shaoyu Su Shaoyu Su Jing Chen Jing Chen |
| author_sort | Qin Zeng |
| collection | DOAJ |
| description | ObjectivesTo investigate the gut microbiota in children with varying degrees of idiopathic short stature (ISS) and to examine the relationship between their intestinal microbiota and lifestyle factors, including diet, exercise, medication, sleep, and psychological state.MethodsA retrospective study involving 58 ISS children was conducted from May to October 2022. Lifestyle data were collected using questionnaires. Fecal samples were analyzed using 16S rRNA gene sequencing to assess microbiota diversity and composition. LEfSe analysis identified differential bacterial communities between ISS-2SD and ISS-3SD groups (LDA score ≥ 2.5). Spearman correlation analysis explored the relationship between microbiota diversity, dominant taxa, and lifestyle factors, visualized in a heatmap.ResultsNo significant differences in alpha diversity of intestinal microbiota were observed among children with varying degrees of short stature at the ISS, but differences were noted in the dominant microbiota. The ISS-2SD group had Leptotrichiaceae and Sneathia as predominant members, whereas the ISS-3SD group was dominated by Lachnoclostridium, Thermous_scotoductus and Thermoles. Correlation analysis revealed that microbiota diversity was linked to diet, especially legume consumption (Shannon index: r=0.372, P=0.004; Simpson index: r=0.379, P=0.003). At the genus level, Prevotella was positively correlated with beverage intake (r=0.262, P=0.047) and sleep quality (r=0.324, P=0.013), while Ezakiella was negatively correlated with meat intake (r=-0.297, P=0.024), Other genera exhibited significant correlations with diet, exercise, and sleep.ConclusionChildren with varying degrees of short stature exhibited differences in their dominant intestinal microbiota. Diet, exercise, and sleep appear to be significant factors influencing these microbial changes in ISS children. |
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| issn | 1664-3224 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj-art-9df61f7c54d84f2c8adfc0057d6ff72c2025-08-01T04:10:21ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-08-011610.3389/fimmu.2025.15667221566722The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective studyQin Zeng0Qin Zeng1Jun Zhu2Jun Zhu3Yanling Hu4Yanling Hu5Shaoyu Su6Shaoyu Su7Jing Chen8Jing Chen9Department of Pediatrics Nursing, West China Second University Hospital, Sichuan University, Chengdu, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaNational Office for Maternal and Child Health Surveillance of China, National Center for Birth Defect Surveillance of China, Department of Pediatrics, West China Second University Hospital, Chengdu, ChinaDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan University, Chengdu, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan University, Chengdu, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan University, Chengdu, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaObjectivesTo investigate the gut microbiota in children with varying degrees of idiopathic short stature (ISS) and to examine the relationship between their intestinal microbiota and lifestyle factors, including diet, exercise, medication, sleep, and psychological state.MethodsA retrospective study involving 58 ISS children was conducted from May to October 2022. Lifestyle data were collected using questionnaires. Fecal samples were analyzed using 16S rRNA gene sequencing to assess microbiota diversity and composition. LEfSe analysis identified differential bacterial communities between ISS-2SD and ISS-3SD groups (LDA score ≥ 2.5). Spearman correlation analysis explored the relationship between microbiota diversity, dominant taxa, and lifestyle factors, visualized in a heatmap.ResultsNo significant differences in alpha diversity of intestinal microbiota were observed among children with varying degrees of short stature at the ISS, but differences were noted in the dominant microbiota. The ISS-2SD group had Leptotrichiaceae and Sneathia as predominant members, whereas the ISS-3SD group was dominated by Lachnoclostridium, Thermous_scotoductus and Thermoles. Correlation analysis revealed that microbiota diversity was linked to diet, especially legume consumption (Shannon index: r=0.372, P=0.004; Simpson index: r=0.379, P=0.003). At the genus level, Prevotella was positively correlated with beverage intake (r=0.262, P=0.047) and sleep quality (r=0.324, P=0.013), while Ezakiella was negatively correlated with meat intake (r=-0.297, P=0.024), Other genera exhibited significant correlations with diet, exercise, and sleep.ConclusionChildren with varying degrees of short stature exhibited differences in their dominant intestinal microbiota. Diet, exercise, and sleep appear to be significant factors influencing these microbial changes in ISS children.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1566722/fullidiopathic short statureintestinal microbiotalifestyle factorscorrelation analysis16S rRNA gene sequencing |
| spellingShingle | Qin Zeng Qin Zeng Jun Zhu Jun Zhu Yanling Hu Yanling Hu Shaoyu Su Shaoyu Su Jing Chen Jing Chen The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study Frontiers in Immunology idiopathic short stature intestinal microbiota lifestyle factors correlation analysis 16S rRNA gene sequencing |
| title | The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study |
| title_full | The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study |
| title_fullStr | The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study |
| title_full_unstemmed | The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study |
| title_short | The critical role of diet, exercise, and sleep in shaping the gut microbiota of children with idiopathic short stature: a Retrospective study |
| title_sort | critical role of diet exercise and sleep in shaping the gut microbiota of children with idiopathic short stature a retrospective study |
| topic | idiopathic short stature intestinal microbiota lifestyle factors correlation analysis 16S rRNA gene sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1566722/full |
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