Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants

Protocol for developing a mouse model of post-primary pulmonary tuberculosis after hematogenous spread in native lungs and lung implants

Summary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung a...

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Bibliographic Details
Main Authors: Shivraj M. Yabaji, Suruchi Lata, Igor Gavrish, Ming Lo, Aoife K. O’Connell, Hans P. Gertje, Colleen E. Thurman, Nicholas A. Crossland, Lester Kobzik, Igor Kramnik
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Immunology
Microbiology
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003909
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Summary:Summary: Here, we present a protocol for a mouse model for studying mechanisms of post-primary pulmonary tuberculosis (PTB) caused by virulent Mycobacterium tuberculosis (Mtb) using subcutaneous hock infection and lung tissue implantation. We describe steps for collagen instillation of lungs, lung and spleen implantation, preparation of Mtb for infection, and hock infection of mice. We then detail procedures for the perfusion of the lung and collection of organs, tissue processing, and histopathologic interpretation.For complete details on the use and execution of this protocol, please refer to Yabaji et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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