OBTAINING IMMUNO REAGENTS FOR SEROLOGICAL DETERMINATION OF DITYLENCHOSIS AGENTS IN AGRICULTURAL AND ORNAMENTAL PLANTS
The purpose of the research is obtaining immuno reagents for determination of two types of parasitic nematodes Ditylenchus destructor and D. dipsaci (antigens and antiserums for them).Materials and methods. Three immunizations of laboratory animals (white scrub mice) have been carried out at a dose...
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| Main Authors: | , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Scientific Centre VIEV
2019-09-01
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| Series: | Российский паразитологический журнал |
| Subjects: | |
| Online Access: | https://vniigis.elpub.ru/jour/article/view/373 |
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| Summary: | The purpose of the research is obtaining immuno reagents for determination of two types of parasitic nematodes Ditylenchus destructor and D. dipsaci (antigens and antiserums for them).Materials and methods. Three immunizations of laboratory animals (white scrub mice) have been carried out at a dose of 20 mcg of the target protein per animal at 7–10 day intervals. The efficient titer of the antiserums obtained was described by the Dot-ELISA method using secondary goat anti-mouse immunoglobulins conjugated with alkaline phosphatase. For measuring a specificity of the obtained immuno reagents, an ELISA plate in an indirect format was used.Results and discussion. The antiserums obtained have a specificity for two types of nematodes D. destructor and D. dipsaci, and for one non-parasitic nematode Rhabditis sp. The specificity of the obtained antiserums ranges from 82 to 97%. The sensitivity of antiserums to homogenates of stem nematodes D. destructor and D. dipsaci of plants at the antiserum dilution 1 : 3000 is 1 ng and 50 ng accordingly. |
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| ISSN: | 1998-8435 2541-7843 |