Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors.
Background. Verification of gastrointestinal stromal tumors and determination of their malignancy potential remain still relevant. Objective. To identify relationships between clinical, histological and immunomorphological (СD117, DOG1, CD34, PDGFR-α, SMA, MSA, desmin, S100, Ki-67, p16, p21) charact...
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State Institution "Dnipro State Medical University"
2015-12-01
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| Online Access: | http://morphology.dma.dp.ua/article/view/139797 |
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| author | V. R. Skoryk |
| author_facet | V. R. Skoryk |
| author_sort | V. R. Skoryk |
| collection | DOAJ |
| description | Background. Verification of gastrointestinal stromal tumors and determination of their malignancy potential remain still relevant. Objective. To identify relationships between clinical, histological and immunomorphological (СD117, DOG1, CD34, PDGFR-α, SMA, MSA, desmin, S100, Ki-67, p16, p21) characteristics of gastrointestinal stromal tumors. Methods. Our study included 50 gastroinestinal stromal tumors, which were divided into subgroups according to clinical (localization), morphological (histological features, morphological variation, the number of mitosis) characteristics depending on the presence of the expression of above mentioned markers. Statistical analysis of data included nonparametric tests. Results. Expression of CD117, DOG1, CD34, PDGFR-α, SMA, desmin, p16, p21 was specified in 94%, 90%, 76%, 61,1%, 24%, 50%, 46,6%, and 47% of cases, respectively. MSA, S100 positive tumors were revealed in 4% and 8% of all cases, and did not let us to divide them into subgroups. The one third of all cases had high expression of Ki-67. Conclusion. Ki-67 is a useful marker for determination of malignancy potential of GIST and significantly correlated with the number of mitosis and cellularity. Ki-67 expression is associated with the presence of p16 staining, it indicates the possibility of using these markers to define malignancy potential of GIST. Markers (CD117, DOG1, CD34, PDGFRα, SMA, p21) did not have prognostic value and were unrelated. There was relationship between the presence of PDGFRα staining and character of CD117 expression. CD117, DOG1 were highly sensitive markers, CD34, PDGFRα were useful markers during verification of GIST, but were less sensitive. Markers SMA, desmin, MSA, S100 are important for the diagnostic process. |
| format | Article |
| id | doaj-art-97c9600aeb9f466bab8a51ff7b603a08 |
| institution | Matheson Library |
| issn | 1997-9665 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | State Institution "Dnipro State Medical University" |
| record_format | Article |
| series | Морфологія |
| spelling | doaj-art-97c9600aeb9f466bab8a51ff7b603a082025-08-02T14:32:49ZengState Institution "Dnipro State Medical University"Морфологія1997-96652015-12-0193748210.26641/1997-9665.2015.3.74-82139797Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors.V. R. Skoryk0ДЗ «Дніпропетровська медична академія МОЗ України»Background. Verification of gastrointestinal stromal tumors and determination of their malignancy potential remain still relevant. Objective. To identify relationships between clinical, histological and immunomorphological (СD117, DOG1, CD34, PDGFR-α, SMA, MSA, desmin, S100, Ki-67, p16, p21) characteristics of gastrointestinal stromal tumors. Methods. Our study included 50 gastroinestinal stromal tumors, which were divided into subgroups according to clinical (localization), morphological (histological features, morphological variation, the number of mitosis) characteristics depending on the presence of the expression of above mentioned markers. Statistical analysis of data included nonparametric tests. Results. Expression of CD117, DOG1, CD34, PDGFR-α, SMA, desmin, p16, p21 was specified in 94%, 90%, 76%, 61,1%, 24%, 50%, 46,6%, and 47% of cases, respectively. MSA, S100 positive tumors were revealed in 4% and 8% of all cases, and did not let us to divide them into subgroups. The one third of all cases had high expression of Ki-67. Conclusion. Ki-67 is a useful marker for determination of malignancy potential of GIST and significantly correlated with the number of mitosis and cellularity. Ki-67 expression is associated with the presence of p16 staining, it indicates the possibility of using these markers to define malignancy potential of GIST. Markers (CD117, DOG1, CD34, PDGFRα, SMA, p21) did not have prognostic value and were unrelated. There was relationship between the presence of PDGFRα staining and character of CD117 expression. CD117, DOG1 were highly sensitive markers, CD34, PDGFRα were useful markers during verification of GIST, but were less sensitive. Markers SMA, desmin, MSA, S100 are important for the diagnostic process.http://morphology.dma.dp.ua/article/view/139797гастроінтестинальні стромальні пухлинидіагностикапотенціал злоякісностіімуногістохімія |
| spellingShingle | V. R. Skoryk Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. Морфологія гастроінтестинальні стромальні пухлини діагностика потенціал злоякісності імуногістохімія |
| title | Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. |
| title_full | Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. |
| title_fullStr | Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. |
| title_full_unstemmed | Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. |
| title_short | Diagnostic and prognostic value of tumorspecific markers (CD117, DOG1, CD34, PDGFR-α), indicators of muscle (SMA, MSA, desmin) and fat (S100) differentiation, expression of Ki-67, p16, p21 in gastrointestinal stromal tumors. |
| title_sort | diagnostic and prognostic value of tumorspecific markers cd117 dog1 cd34 pdgfr α indicators of muscle sma msa desmin and fat s100 differentiation expression of ki 67 p16 p21 in gastrointestinal stromal tumors |
| topic | гастроінтестинальні стромальні пухлини діагностика потенціал злоякісності імуногістохімія |
| url | http://morphology.dma.dp.ua/article/view/139797 |
| work_keys_str_mv | AT vrskoryk diagnosticandprognosticvalueoftumorspecificmarkerscd117dog1cd34pdgfraindicatorsofmusclesmamsadesminandfats100differentiationexpressionofki67p16p21ingastrointestinalstromaltumors |