Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM
Summary: Gram-negative bacteria possess an outer membrane (OM) that acts as a barrier against toxic compounds. Lipopolysaccharide (LPS) in the outer leaflet of the OM is crucial for barrier function and is transported to the OM by the LPS transport system. The LPS translocon (the LptDE complex), med...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725007843 |
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| author | Ryoji Miyazaki Mai Kimoto Hidetaka Kohga Tomoya Tsukazaki |
| author_facet | Ryoji Miyazaki Mai Kimoto Hidetaka Kohga Tomoya Tsukazaki |
| author_sort | Ryoji Miyazaki |
| collection | DOAJ |
| description | Summary: Gram-negative bacteria possess an outer membrane (OM) that acts as a barrier against toxic compounds. Lipopolysaccharide (LPS) in the outer leaflet of the OM is crucial for barrier function and is transported to the OM by the LPS transport system. The LPS translocon (the LptDE complex), mediates LPS assembly into the OM. Recently, the small lipoprotein LptM was identified as an LptDE interactor that facilitates LptD maturation. However, its mechanism remains unclear. Here, we investigate the detailed interaction between LptM and LptD. We find that LptM interacts with the folded LptD intermediate at the late stage of its maturation. Mutational analyses demonstrate that the N-terminal conserved region of LptM is essential for its function. Cryoelectron microscopy structural analysis of the Escherichia coli LptDEM complex, combined with biochemical analyses, reveals the molecular basis of the LptM-LptD interaction and its functional importance. Thus, we propose that LptM stabilizes LptD for proper assembly. |
| format | Article |
| id | doaj-art-94e3b5a02470461ab923306fdbc5562c |
| institution | Matheson Library |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-94e3b5a02470461ab923306fdbc5562c2025-07-17T04:44:17ZengElsevierCell Reports2211-12472025-08-01448116013Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptMRyoji Miyazaki0Mai Kimoto1Hidetaka Kohga2Tomoya Tsukazaki3Nara Institute of Science and Technology, Nara, Japan; Corresponding authorNara Institute of Science and Technology, Nara, JapanNara Institute of Science and Technology, Nara, JapanNara Institute of Science and Technology, Nara, Japan; Corresponding authorSummary: Gram-negative bacteria possess an outer membrane (OM) that acts as a barrier against toxic compounds. Lipopolysaccharide (LPS) in the outer leaflet of the OM is crucial for barrier function and is transported to the OM by the LPS transport system. The LPS translocon (the LptDE complex), mediates LPS assembly into the OM. Recently, the small lipoprotein LptM was identified as an LptDE interactor that facilitates LptD maturation. However, its mechanism remains unclear. Here, we investigate the detailed interaction between LptM and LptD. We find that LptM interacts with the folded LptD intermediate at the late stage of its maturation. Mutational analyses demonstrate that the N-terminal conserved region of LptM is essential for its function. Cryoelectron microscopy structural analysis of the Escherichia coli LptDEM complex, combined with biochemical analyses, reveals the molecular basis of the LptM-LptD interaction and its functional importance. Thus, we propose that LptM stabilizes LptD for proper assembly.http://www.sciencedirect.com/science/article/pii/S2211124725007843CP: Molecular biologyCP: Microbiology |
| spellingShingle | Ryoji Miyazaki Mai Kimoto Hidetaka Kohga Tomoya Tsukazaki Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM Cell Reports CP: Molecular biology CP: Microbiology |
| title | Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| title_full | Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| title_fullStr | Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| title_full_unstemmed | Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| title_short | Structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein LptM |
| title_sort | structural basis of lipopolysaccharide translocon assembly mediated by the small lipoprotein lptm |
| topic | CP: Molecular biology CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725007843 |
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