Cloning of coat protein gene of Grapevine fanleaf virus Hangzhou isolate and construction of its plant expression vector
The coat protein (CP) gene cDNA of Grapevine fanleaf virus (GFLV) Hangzhou isolate was amplified from total viral RNA by RT-PCR, then inserted into pGEM-T-easy Vector. Sequence analysis showed that this gene contained 1515 nucleotide acids encoding 504 amino acids. The nucleotide sequence and deduce...
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| Format: | Artikel |
| Sprache: | Englisch |
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Zhejiang University Press
2002-11-01
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| Schriftenreihe: | 浙江大学学报. 农业与生命科学版 |
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| Online-Zugang: | https://www.academax.com/doi/10.3785/1008-9209.2002.06.0646 |
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| Zusammenfassung: | The coat protein (CP) gene cDNA of Grapevine fanleaf virus (GFLV) Hangzhou isolate was amplified from total viral RNA by RT-PCR, then inserted into pGEM-T-easy Vector. Sequence analysis showed that this gene contained 1515 nucleotide acids encoding 504 amino acids. The nucleotide sequence and deduced amino acid sequence of GFLV-H CP shared, respectively, 88% and 95% identity to those of GFLV-F13. The gene of GFLV-H CP was cloned into plant expression vector pBI121, which was transferred into Agrobacterium tumefaciens LBA4404 by triparental mating. |
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| ISSN: | 1008-9209 2097-5155 |