Screening of high chitinase-producing stains from Trichoderma harzianum and conditions for enzyme production
Trichoderma harzianum wild-type strains were implanted by N<sup>+</sup> to produce mutagenesis. A mutant stain H-13 with higher activity of chitinase was obtained by screening the mutants with transparent zone, and analyzing chitinase activity by DNS method. Single-factor design and orth...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.02.005 |
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| Summary: | Trichoderma harzianum wild-type strains were implanted by N<sup>+</sup> to produce mutagenesis. A mutant stain H-13 with higher activity of chitinase was obtained by screening the mutants with transparent zone, and analyzing chitinase activity by DNS method. Single-factor design and orthogonal test design were used for optimizing cultural conditions of chitinase production from mutant strain H-13. Single-factor experimentation showed that the media added 1.0% peptone led to highest chitinase production, and 1.0% colloidal chitin was the best media for inducing chitinase. The mutant strain showed higher enzymatic activity in this media with initial pH 5.5 for 96 h of culture. Orthogonal test showed that chitinase activity of strain H-13 could reach 731.69 U·mL<sup>-1</sup> cultured in the optimal medium with 1.0% peptone, 0.5% colloidal chitin and initial pH 5.0 for 96 h, which was one fold increase compared to the wild strain enzyme vigor. |
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| ISSN: | 1008-9209 2097-5155 |