Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability
In two experiments, the effects of succinate and NADH (reduced nicotinamide adenine dinucleotide) on metmyoglobin reductase activity and electron transport chain-linked metmyoglobin reduction were investigated and compared. In experiment 1, metmyoglobin (MetMb), substrate and inhibitors were incubat...
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KeAi Communications Co., Ltd.
2014-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914607541 |
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| author | Xiao-guang GAO Zhen-yu WANG Meng-tian TANG Chang-wei MA Rui-tong DAI |
| author_facet | Xiao-guang GAO Zhen-yu WANG Meng-tian TANG Chang-wei MA Rui-tong DAI |
| author_sort | Xiao-guang GAO |
| collection | DOAJ |
| description | In two experiments, the effects of succinate and NADH (reduced nicotinamide adenine dinucleotide) on metmyoglobin reductase activity and electron transport chain-linked metmyoglobin reduction were investigated and compared. In experiment 1, metmyoglobin (MetMb), substrate and inhibitors were incubated with mitochondria. Comparsion of the effects of succinate and NADH on MetMb reduction was investigated. The MetMb percentage in sample treated with 8 mol L−1 succinate decreased by about 69% after 3 h incubation, and the effect was inhibited by the addition of 10 mol L−1 electron transfer chain complex II inhibitor malonic acid; the MetMb percentage in samples treated with 2 mol L−1 NADH decreased by 56% and the effect was inhibited by the addition of 0.02 mol L−1 electron transport chain complex I inhibitor rotenone. These results indicated that electron transport chain played an important role in MetMb reduction. Both complex II and complex I take part in the MetMb reduction in mitochondria through different pathways. NADH-MetMb reduction system was less stable than succinate-MetMb system. In experiment 2, the beef longissimus dorsi muscle was blended with different concentrations of succinate or NADH. Enhancing patties with higher concentration of succinate or NADH improved colour stability in vacuum packaged samples (P<0.05). These results verified that mitochondria electron transport chain is related to the MetMb reduction in meat system. |
| format | Article |
| id | doaj-art-8cb298f55e3e4db7b51c11bc672c4d6b |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2014-08-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Journal of Integrative Agriculture |
| spelling | doaj-art-8cb298f55e3e4db7b51c11bc672c4d6b2025-08-02T13:28:19ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192014-08-0113818171826Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour StabilityXiao-guang GAO0Zhen-yu WANG1Meng-tian TANG2Chang-wei MA3Rui-tong DAI4College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.China; College of Biological Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050000, P.R.China; Correspondence DAI Rui-tong, Tel: +86-10-62737644, Mobile: 15376776069, Fax: +86-10-62839300Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, P.R.China; Correspondence DAI Rui-tong, Tel: +86-10-62737644, Mobile: 15376776069, Fax: +86-10-62839300In two experiments, the effects of succinate and NADH (reduced nicotinamide adenine dinucleotide) on metmyoglobin reductase activity and electron transport chain-linked metmyoglobin reduction were investigated and compared. In experiment 1, metmyoglobin (MetMb), substrate and inhibitors were incubated with mitochondria. Comparsion of the effects of succinate and NADH on MetMb reduction was investigated. The MetMb percentage in sample treated with 8 mol L−1 succinate decreased by about 69% after 3 h incubation, and the effect was inhibited by the addition of 10 mol L−1 electron transfer chain complex II inhibitor malonic acid; the MetMb percentage in samples treated with 2 mol L−1 NADH decreased by 56% and the effect was inhibited by the addition of 0.02 mol L−1 electron transport chain complex I inhibitor rotenone. These results indicated that electron transport chain played an important role in MetMb reduction. Both complex II and complex I take part in the MetMb reduction in mitochondria through different pathways. NADH-MetMb reduction system was less stable than succinate-MetMb system. In experiment 2, the beef longissimus dorsi muscle was blended with different concentrations of succinate or NADH. Enhancing patties with higher concentration of succinate or NADH improved colour stability in vacuum packaged samples (P<0.05). These results verified that mitochondria electron transport chain is related to the MetMb reduction in meat system.http://www.sciencedirect.com/science/article/pii/S2095311914607541succinateNADHmalonic acidrotenonemetmyoglobinelectron transport chain |
| spellingShingle | Xiao-guang GAO Zhen-yu WANG Meng-tian TANG Chang-wei MA Rui-tong DAI Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability Journal of Integrative Agriculture succinate NADH malonic acid rotenone metmyoglobin electron transport chain |
| title | Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability |
| title_full | Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability |
| title_fullStr | Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability |
| title_full_unstemmed | Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability |
| title_short | Comparsion of the Effects of Succinate and NADH on Postmortem Metmyoglobin Redcutase Activity and Beef Colour Stability |
| title_sort | comparsion of the effects of succinate and nadh on postmortem metmyoglobin redcutase activity and beef colour stability |
| topic | succinate NADH malonic acid rotenone metmyoglobin electron transport chain |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914607541 |
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