Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation
Sweet potato (<i>Ipomoea batatas</i> (L.) Lam.), as an important crop, is rich in polyphenols, vitamins, minerals, and other nutrients in its roots and leaves and is gradually gaining popularity. The use of endophytic bacteria to improve the quality of sweet potato can protect the enviro...
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2025-06-01
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| author | Yiming Wang Jingwen Hao Jingsheng Gu Jiaying Wu Yongjing Zhang Ting Liang Haimeng Bai Qinghe Cao Jihong Jiang Ludan Li Xiaoying Cao |
| author_facet | Yiming Wang Jingwen Hao Jingsheng Gu Jiaying Wu Yongjing Zhang Ting Liang Haimeng Bai Qinghe Cao Jihong Jiang Ludan Li Xiaoying Cao |
| author_sort | Yiming Wang |
| collection | DOAJ |
| description | Sweet potato (<i>Ipomoea batatas</i> (L.) Lam.), as an important crop, is rich in polyphenols, vitamins, minerals, and other nutrients in its roots and leaves and is gradually gaining popularity. The use of endophytic bacteria to improve the quality of sweet potato can protect the environment and effectively promote the sustainable development of the sweet potato industry. In this study, 12 strains of endophytic bacteria were isolated from sweet potato. Through nitrogen fixation, phosphorus solubilization, indoleacetic acid production, siderophore production, ACC deaminase production, and carboxymethyl cellulose production, three strains with multiple biological activities were screened out. Among them, MEPW12 had the most plant growth-promoting functions. In addition, MEPW12 promoted host chlorophyll accumulation and inhibited pathogen growth and colonization in sweet potato roots and can utilize various carbon sources and salts for growth. It can also grow in extreme environments of high salt and weak acid. MEPW12 was identified as <i>Bacillus amyloliquefaciens</i> with a genome size of 3,928,046 bp and a GC content of 46.59%. After the annotation of multiple databases, it was found that MEPW12 had multiple enzymatic activities and metabolic potential. Comparative genomics and pan-genomics analyses revealed that other <i>Bacillus</i> sp. strains of MEPW12 have similar functions. However, due to adaptation to different growth environments, there are still genomic differences and changes. Inoculation with MEPW12 induced the high expression of <i>IbGH3.10</i>, <i>IbERF1</i>, and other genes, thereby promoting the growth of sweet potatoes. <i>Bacillus amyloliquefaciens</i> strain MEPW12 is a sweet potato endophyte with multiple growth-promoting functions, which can promote the growth of sweet potato seedlings. This study provides new microbial resources for developing microbial agents and improving the quality of sweet potatoes. |
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| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-8c4837cd08384e7c813d936b4a22bbd02025-06-25T14:12:32ZengMDPI AGMicroorganisms2076-26072025-06-01136132210.3390/microorganisms13061322Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato CultivationYiming Wang0Jingwen Hao1Jingsheng Gu2Jiaying Wu3Yongjing Zhang4Ting Liang5Haimeng Bai6Qinghe Cao7Jihong Jiang8Ludan Li9Xiaoying Cao10The Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaThe Key Laboratory of Biotechnology for Medicinal and Edible Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, ChinaSweet potato (<i>Ipomoea batatas</i> (L.) Lam.), as an important crop, is rich in polyphenols, vitamins, minerals, and other nutrients in its roots and leaves and is gradually gaining popularity. The use of endophytic bacteria to improve the quality of sweet potato can protect the environment and effectively promote the sustainable development of the sweet potato industry. In this study, 12 strains of endophytic bacteria were isolated from sweet potato. Through nitrogen fixation, phosphorus solubilization, indoleacetic acid production, siderophore production, ACC deaminase production, and carboxymethyl cellulose production, three strains with multiple biological activities were screened out. Among them, MEPW12 had the most plant growth-promoting functions. In addition, MEPW12 promoted host chlorophyll accumulation and inhibited pathogen growth and colonization in sweet potato roots and can utilize various carbon sources and salts for growth. It can also grow in extreme environments of high salt and weak acid. MEPW12 was identified as <i>Bacillus amyloliquefaciens</i> with a genome size of 3,928,046 bp and a GC content of 46.59%. After the annotation of multiple databases, it was found that MEPW12 had multiple enzymatic activities and metabolic potential. Comparative genomics and pan-genomics analyses revealed that other <i>Bacillus</i> sp. strains of MEPW12 have similar functions. However, due to adaptation to different growth environments, there are still genomic differences and changes. Inoculation with MEPW12 induced the high expression of <i>IbGH3.10</i>, <i>IbERF1</i>, and other genes, thereby promoting the growth of sweet potatoes. <i>Bacillus amyloliquefaciens</i> strain MEPW12 is a sweet potato endophyte with multiple growth-promoting functions, which can promote the growth of sweet potato seedlings. This study provides new microbial resources for developing microbial agents and improving the quality of sweet potatoes.https://www.mdpi.com/2076-2607/13/6/1322endophytesweet potatogrowth promoting bacteriagenomepan-genomic analysis |
| spellingShingle | Yiming Wang Jingwen Hao Jingsheng Gu Jiaying Wu Yongjing Zhang Ting Liang Haimeng Bai Qinghe Cao Jihong Jiang Ludan Li Xiaoying Cao Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation Microorganisms endophyte sweet potato growth promoting bacteria genome pan-genomic analysis |
| title | Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation |
| title_full | Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation |
| title_fullStr | Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation |
| title_full_unstemmed | Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation |
| title_short | Genome-Driven Functional Validation of <i>Bacillus amyloliquefaciens</i> Strain MEPW12: A Multifunctional Endophyte for Sustainable Sweet Potato Cultivation |
| title_sort | genome driven functional validation of i bacillus amyloliquefaciens i strain mepw12 a multifunctional endophyte for sustainable sweet potato cultivation |
| topic | endophyte sweet potato growth promoting bacteria genome pan-genomic analysis |
| url | https://www.mdpi.com/2076-2607/13/6/1322 |
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