Plant expression vector's construction of VP2 gene of infectious bursal disease virus
The VP2 gene of infectious bursal disease virus (IBDV, HZ96 strain) was amplified by polymerase chain reaction with special primers containing Kpn Ⅰ and Sal Ⅰ. The purified PCR product was digested by restricted enzymes Kpn Ⅰ and Sal Ⅰ. The digested and purified VP2 gene of infectious bursal disease...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2003-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0644 |
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| _version_ | 1839607213233012736 |
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| author | WU Jian-xiang YU Lian GONG Hui |
| author_facet | WU Jian-xiang YU Lian GONG Hui |
| author_sort | WU Jian-xiang |
| collection | DOAJ |
| description | The VP2 gene of infectious bursal disease virus (IBDV, HZ96 strain) was amplified by polymerase chain reaction with special primers containing Kpn Ⅰ and Sal Ⅰ. The purified PCR product was digested by restricted enzymes Kpn Ⅰ and Sal Ⅰ. The digested and purified VP2 gene of infectious bursal disease virus was cloned into plant expression vector Cambia1301 that contains rice endosperm-specific promoter GT1. The result shows that Cambia1301 with VP2 gene was successfully transferred into Agrobacterium tumefaciens (Aotumefaciens) by method of triparental mating. |
| format | Article |
| id | doaj-art-8bc7bcc19f424f849b5ec1ba8379c7d9 |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-8bc7bcc19f424f849b5ec1ba8379c7d92025-08-01T05:39:45ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-11-012964464810.3785/1008-9209.2003.06.064410089209Plant expression vector's construction of VP2 gene of infectious bursal disease virusWU Jian-xiangYU LianGONG HuiThe VP2 gene of infectious bursal disease virus (IBDV, HZ96 strain) was amplified by polymerase chain reaction with special primers containing Kpn Ⅰ and Sal Ⅰ. The purified PCR product was digested by restricted enzymes Kpn Ⅰ and Sal Ⅰ. The digested and purified VP2 gene of infectious bursal disease virus was cloned into plant expression vector Cambia1301 that contains rice endosperm-specific promoter GT1. The result shows that Cambia1301 with VP2 gene was successfully transferred into Agrobacterium tumefaciens (Aotumefaciens) by method of triparental mating.https://www.academax.com/doi/10.3785/1008-9209.2003.06.0644infectious bursal disease virusVP2 geneplant expression vectorrice endosperm-specific promoter GT1 |
| spellingShingle | WU Jian-xiang YU Lian GONG Hui Plant expression vector's construction of VP2 gene of infectious bursal disease virus 浙江大学学报. 农业与生命科学版 infectious bursal disease virus VP2 gene plant expression vector rice endosperm-specific promoter GT1 |
| title | Plant expression vector's construction of VP2 gene of infectious bursal disease virus |
| title_full | Plant expression vector's construction of VP2 gene of infectious bursal disease virus |
| title_fullStr | Plant expression vector's construction of VP2 gene of infectious bursal disease virus |
| title_full_unstemmed | Plant expression vector's construction of VP2 gene of infectious bursal disease virus |
| title_short | Plant expression vector's construction of VP2 gene of infectious bursal disease virus |
| title_sort | plant expression vector s construction of vp2 gene of infectious bursal disease virus |
| topic | infectious bursal disease virus VP2 gene plant expression vector rice endosperm-specific promoter GT1 |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0644 |
| work_keys_str_mv | AT wujianxiang plantexpressionvectorsconstructionofvp2geneofinfectiousbursaldiseasevirus AT yulian plantexpressionvectorsconstructionofvp2geneofinfectiousbursaldiseasevirus AT gonghui plantexpressionvectorsconstructionofvp2geneofinfectiousbursaldiseasevirus |