Mutagenic improvement of Bacillus cereus resistant to Ralstonia solanacearum
To improve the resistance of Bacillus cereus to Ralstonia solanacearum, an antagonistic bacterium belonging to B. cereus, named as L<sub>1</sub>, was isolated from the ginger-growing soil, which was resistance to R. solanacearum at some degree. Ultraviolet rays (UV), ethyl methane sulfon...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.02.006 |
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| Summary: | To improve the resistance of Bacillus cereus to Ralstonia solanacearum, an antagonistic bacterium belonging to B. cereus, named as L<sub>1</sub>, was isolated from the ginger-growing soil, which was resistance to R. solanacearum at some degree. Ultraviolet rays (UV), ethyl methane sulfonate (EMS) and acridine orange (AO) were used as mutagens to treat strain L<sub>1</sub>, and then the mutants were identified and screened. The results showed that the antagonistic abilities of mutant strains were significantly enhanced compared with the original strain L<sub>1</sub>, and the concentrations of antagonistic protein of mutant-strains EMS-2 and UL-1, were 30.38% and 29.18% higher than that of original strain L<sub>1</sub> separately, being significant difference between the two mutant-strains and L<sub>1</sub>. In addition, strain L<sub>1</sub> was sensitive to UV especially during the period of 60 s, and up to 90 s, the utmost positive mutation rate was detected (24.8%). However, no positive mutants were founded when the strain L<sub>1</sub> was treated with AO. Moreover, the lethal rate of strain L<sub>1</sub> was up to 90% with the treatment of EMS beyond 80 min, indicating that to obtain the more number of positive mutants, the lower dosage of EMS (0.16 mol· mL<sup>-1</sup>) for longer treatment time (60 min) was the possible method of mutant treatment. |
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| ISSN: | 1008-9209 2097-5155 |