Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent
Aim: Zinc is essential for normal bone growth and can promote bone regeneration. Processed human bone allograft treated with zinc shows improved bone formation activity. Various factors were tested for effects on zinc binding to bone allograft with the long-term goal of developing methods to enhance...
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| Format: | Article |
| Language: | English |
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Open Exploration Publishing Inc.
2025-07-01
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| Series: | Exploration of BioMat-X |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A101341/101341.pdf |
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| author | Thomas Helbig James F. Thornton Darian A. Napoleon Luke G. Menken John Flacco Joseph Miceli Kyle Auger Deboleena Kanjilal Maya Deza Culbertson Sheldon Lin Joseph Benevenia J. Patrick O’Connor |
| author_facet | Thomas Helbig James F. Thornton Darian A. Napoleon Luke G. Menken John Flacco Joseph Miceli Kyle Auger Deboleena Kanjilal Maya Deza Culbertson Sheldon Lin Joseph Benevenia J. Patrick O’Connor |
| author_sort | Thomas Helbig |
| collection | DOAJ |
| description | Aim: Zinc is essential for normal bone growth and can promote bone regeneration. Processed human bone allograft treated with zinc shows improved bone formation activity. Various factors were tested for effects on zinc binding to bone allograft with the long-term goal of developing methods to enhance the bone formation activity and safety of bone allograft in orthopaedic applications. Methods: The amount of zinc bound to allograft was measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Fluorescent visualization of zinc bound to allograft was accomplished using Zinpyr-1. The potential anti-microbial property of zinc-treated allograft was measured by exposing allograft to Staphylococcus aureus. After washing, the exposed allograft was cultured in bacterial media to measure residual Staphylococcus aureus. Data were analyzed using standard parametric methods. Results: Rapid binding of zinc to bone allograft (1–15 min) was relatively insensitive to zinc concentration, incubation time, pH, or divalent cation competition. In contrast, zinc salt counter ions had significant effects, with zinc acetate producing more rapid zinc binding than zinc chloride or zinc picolinate. The ability of Staphylococcus aureus to contaminate bone allograft was also significantly reduced by prior zinc treatment. Conclusions: The study results provide guidelines for modifying the processing of bone allograft to enhance bone formation activity while also improving the resistance of the allograft to bacterial contamination. |
| format | Article |
| id | doaj-art-8b0c48c77af44b67a0a77c9d742d73e8 |
| institution | Matheson Library |
| issn | 2996-9476 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of BioMat-X |
| spelling | doaj-art-8b0c48c77af44b67a0a77c9d742d73e82025-07-15T01:58:30ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762025-07-01234653. French10.37349/ebmx.2025.101341Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agentThomas Helbig0James F. Thornton1https://orcid.org/0009-0009-5581-9498Darian A. Napoleon2https://orcid.org/0009-0001-7660-9758Luke G. Menken3https://orcid.org/0000-0002-0397-2951John Flacco4https://orcid.org/0000-0003-0086-0740Joseph Miceli5Kyle Auger6https://orcid.org/0009-0009-0000-6342Deboleena Kanjilal7Maya Deza Culbertson8https://orcid.org/0000-0001-6463-414XSheldon Lin9https://orcid.org/0000-0002-6639-428XJoseph Benevenia10J. Patrick O’Connor11https://orcid.org/0000-0003-0282-1104Rutgers-Robert Wood Johnson Medical School, Piscataway, NJ 08855, USA; Current address: Department of Surgery, Morristown Medical Center, Morristown, NJ 07960, USARutgers Biomedical Health Sciences School of Graduate Studies, Newark, NJ 07103, USA; Current address: Rutgers-New Jersey Medical School, Newark, NJ 07103, USARutgers Biomedical Health Sciences School of Graduate Studies, Newark, NJ 07103, USA; Current address: Rutgers-New Jersey Medical School, Newark, NJ 07103, USADepartment of Orthopaedic Surgery, Jersey City Medical Center, Jersey City, NJ 07302, USADepartment of Orthopaedic Surgery, Jersey City Medical Center, Jersey City, NJ 07302, USADepartment of Orthopaedic Surgery, Jersey City Medical Center, Jersey City, NJ 07302, USADepartment of Orthopaedic Surgery, Jersey City Medical Center, Jersey City, NJ 07302, USADepartment of Orthopaedics, Rutgers-New Jersey Medical School, Newark, NJ 07103, USA; Current address: Shift Health, Toronto, ON M5R 3N5, CanadaDepartment of Orthopaedics, Rutgers-New Jersey Medical School, Newark, NJ 07103, USARutgers Biomedical Health Sciences School of Graduate Studies, Newark, NJ 07103, USA; Department of Orthopaedics, Rutgers-New Jersey Medical School, Newark, NJ 07103, USARutgers Biomedical Health Sciences School of Graduate Studies, Newark, NJ 07103, USA; Department of Orthopaedics, Rutgers-New Jersey Medical School, Newark, NJ 07103, USARutgers Biomedical Health Sciences School of Graduate Studies, Newark, NJ 07103, USA; Department of Orthopaedics, Rutgers-New Jersey Medical School, Newark, NJ 07103, USAAim: Zinc is essential for normal bone growth and can promote bone regeneration. Processed human bone allograft treated with zinc shows improved bone formation activity. Various factors were tested for effects on zinc binding to bone allograft with the long-term goal of developing methods to enhance the bone formation activity and safety of bone allograft in orthopaedic applications. Methods: The amount of zinc bound to allograft was measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). Fluorescent visualization of zinc bound to allograft was accomplished using Zinpyr-1. The potential anti-microbial property of zinc-treated allograft was measured by exposing allograft to Staphylococcus aureus. After washing, the exposed allograft was cultured in bacterial media to measure residual Staphylococcus aureus. Data were analyzed using standard parametric methods. Results: Rapid binding of zinc to bone allograft (1–15 min) was relatively insensitive to zinc concentration, incubation time, pH, or divalent cation competition. In contrast, zinc salt counter ions had significant effects, with zinc acetate producing more rapid zinc binding than zinc chloride or zinc picolinate. The ability of Staphylococcus aureus to contaminate bone allograft was also significantly reduced by prior zinc treatment. Conclusions: The study results provide guidelines for modifying the processing of bone allograft to enhance bone formation activity while also improving the resistance of the allograft to bacterial contamination.https://www.explorationpub.com/uploads/Article/A101341/101341.pdfbone regenerationbone allograftzincanti-microbialstaphylococcus aureus |
| spellingShingle | Thomas Helbig James F. Thornton Darian A. Napoleon Luke G. Menken John Flacco Joseph Miceli Kyle Auger Deboleena Kanjilal Maya Deza Culbertson Sheldon Lin Joseph Benevenia J. Patrick O’Connor Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent Exploration of BioMat-X bone regeneration bone allograft zinc anti-microbial staphylococcus aureus |
| title | Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent |
| title_full | Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent |
| title_fullStr | Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent |
| title_full_unstemmed | Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent |
| title_short | Binding of zinc to processed human bone allograft and potential use of zinc as an anti-microbial agent |
| title_sort | binding of zinc to processed human bone allograft and potential use of zinc as an anti microbial agent |
| topic | bone regeneration bone allograft zinc anti-microbial staphylococcus aureus |
| url | https://www.explorationpub.com/uploads/Article/A101341/101341.pdf |
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