RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.)
Generic diversity (GD) screening helps to identify candidate genes involved in diseases, pests, or other stress resistance in plants. GD is crucial for maintaining vigor and healthy plant population. The random amplified polymorphic DNA (RAPD) is a rapid polymerase chain reaction (PCR)-based molecul...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2023-03-01
|
| Series: | Plant Trends |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=184389 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839617180530900992 |
|---|---|
| author | Rokibul Hasan Md Atikur Rahman Ahmad Humayan Kabir F M Ali Haydar Md Abu Reza |
| author_facet | Rokibul Hasan Md Atikur Rahman Ahmad Humayan Kabir F M Ali Haydar Md Abu Reza |
| author_sort | Rokibul Hasan |
| collection | DOAJ |
| description | Generic diversity (GD) screening helps to identify candidate genes involved in diseases, pests, or other stress resistance in plants. GD is crucial for maintaining vigor and healthy plant population. The random amplified polymorphic DNA (RAPD) is a rapid polymerase chain reaction (PCR)-based molecular marker, effective for screening genetic diversity in diverse plant species. In this study, we applied the RAPD-markers-assisted PCR approach to explore genetic diversity (GD) among 7 varieties of eggplant (Solanum melongena L.) at DNA level. Further, we assessed the presence of Bt-Cry1Ac gene in those varieties using the PCR approach. The eggplants showed genetic diversity at the DNA level. The amplification with two RAPD primers (OPA-02, OPA-04) produced a total of 41 bands. The highest similarity showed 78.57% among sample 4 (Eggplant China) vs sample 7 (Purple king F1) and sample 1 (Eggplant Srilanka) vs sample 4 (Eggplant China), while the lowest was 28.57% among sample 7 (Purple king F1) vs sample 3 (Eggplant Chittagong-1). In addition, the PCR results for the Bt Cry1Ac primer did not show a considerable band in the expected amplicon size. The dendrogram showed the genetic variability and relationship among all seven eggplant varieties at the genetic level. The study formulated a simple marker-based method for an effective molecular protocol to study genetics in eggplants. It also reported the presence of the Bt gene in popular eggplant varieties. [Plant Trends 2023; 1(1.000): 20-30] |
| format | Article |
| id | doaj-art-8abd2a3a94e1407ca5b540d2f435079f |
| institution | Matheson Library |
| issn | 3006-5658 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Plant Trends |
| spelling | doaj-art-8abd2a3a94e1407ca5b540d2f435079f2025-07-25T03:33:57ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyPlant Trends3006-56582023-03-0111203010.5455/pt.2023.03184389RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.)Rokibul Hasan0Md Atikur Rahman1Ahmad Humayan Kabir2F M Ali Haydar3Md Abu Reza4Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh; School of Sciences, University of Louisiana at Monroe, Monroe, LA 71209, USAGrassland and Forage Division, National Institute of Animal Science, Rural Development, Administration, Cheonan 31000, Republic of KoreaSchool of Sciences, University of Louisiana at Monroe, Monroe, LA 71209, USADepartment of Botany, University of Rajshahi, Rajshahi, 6205, BangladeshDepartment of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, BangladeshGeneric diversity (GD) screening helps to identify candidate genes involved in diseases, pests, or other stress resistance in plants. GD is crucial for maintaining vigor and healthy plant population. The random amplified polymorphic DNA (RAPD) is a rapid polymerase chain reaction (PCR)-based molecular marker, effective for screening genetic diversity in diverse plant species. In this study, we applied the RAPD-markers-assisted PCR approach to explore genetic diversity (GD) among 7 varieties of eggplant (Solanum melongena L.) at DNA level. Further, we assessed the presence of Bt-Cry1Ac gene in those varieties using the PCR approach. The eggplants showed genetic diversity at the DNA level. The amplification with two RAPD primers (OPA-02, OPA-04) produced a total of 41 bands. The highest similarity showed 78.57% among sample 4 (Eggplant China) vs sample 7 (Purple king F1) and sample 1 (Eggplant Srilanka) vs sample 4 (Eggplant China), while the lowest was 28.57% among sample 7 (Purple king F1) vs sample 3 (Eggplant Chittagong-1). In addition, the PCR results for the Bt Cry1Ac primer did not show a considerable band in the expected amplicon size. The dendrogram showed the genetic variability and relationship among all seven eggplant varieties at the genetic level. The study formulated a simple marker-based method for an effective molecular protocol to study genetics in eggplants. It also reported the presence of the Bt gene in popular eggplant varieties. [Plant Trends 2023; 1(1.000): 20-30]http://www.ejmanager.com/fulltextpdf.php?mno=184389brinjalbacillus thuringiensisvarietal differentiationprotein polymorphismmarker-assisted breeding |
| spellingShingle | Rokibul Hasan Md Atikur Rahman Ahmad Humayan Kabir F M Ali Haydar Md Abu Reza RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) Plant Trends brinjal bacillus thuringiensis varietal differentiation protein polymorphism marker-assisted breeding |
| title | RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) |
| title_full | RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) |
| title_fullStr | RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) |
| title_full_unstemmed | RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) |
| title_short | RAPD-markers assisted genetic diversity analysis and Bt-Cry1Ac gene identification in eggplant (Solanum melongena L.) |
| title_sort | rapd markers assisted genetic diversity analysis and bt cry1ac gene identification in eggplant solanum melongena l |
| topic | brinjal bacillus thuringiensis varietal differentiation protein polymorphism marker-assisted breeding |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=184389 |
| work_keys_str_mv | AT rokibulhasan rapdmarkersassistedgeneticdiversityanalysisandbtcry1acgeneidentificationineggplantsolanummelongenal AT mdatikurrahman rapdmarkersassistedgeneticdiversityanalysisandbtcry1acgeneidentificationineggplantsolanummelongenal AT ahmadhumayankabir rapdmarkersassistedgeneticdiversityanalysisandbtcry1acgeneidentificationineggplantsolanummelongenal AT fmalihaydar rapdmarkersassistedgeneticdiversityanalysisandbtcry1acgeneidentificationineggplantsolanummelongenal AT mdabureza rapdmarkersassistedgeneticdiversityanalysisandbtcry1acgeneidentificationineggplantsolanummelongenal |