Characterization and Expression Analysis of PUB Gene Family Involved in Drought Stress Response in Trifoliate Orange (<i>Poncirus trifoliata</i>)

Characterization and Expression Analysis of PUB Gene Family Involved in Drought Stress Response in Trifoliate Orange (<i>Poncirus trifoliata</i>)

The U-box E3 ubiquitin ligase (PUB) gene family plays an important role in regulating plant responses to abiotic stress. <i>Poncirus trifoliata</i> (trifoliate orange), a citrus rootstock with notable cold, drought, and salt tolerance, serves as an excellent model for studying stress-res...

Full description

Saved in:
Bibliographic Details
Main Authors: Bobo Song, Sanpeng Jin, Xuchen Gong, Yong Liu, Dechun Liu, Li Yang, Wei Hu, Liuqing Kuang, Jie Song
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Horticulturae
Subjects:
<i>Poncirus trifoliata</i>
<i>PUB</i>
synteny analysis
gene expression
drought stress
Online Access:https://www.mdpi.com/2311-7524/11/6/604
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The U-box E3 ubiquitin ligase (PUB) gene family plays an important role in regulating plant responses to abiotic stress. <i>Poncirus trifoliata</i> (trifoliate orange), a citrus rootstock with notable cold, drought, and salt tolerance, serves as an excellent model for studying stress-responsive genes. In this study, a total of 47 PUB genes (<i>PtrPUBs</i>) were identified in the trifoliate orange genome. Chromosomal distribution analysis indicated that <i>PtrPUB</i> genes were unevenly distributed across nine trifoliate orange chromosomes. Phylogenetic tree analysis indicated that 170 PUB proteins from trifoliate orange, <i>Arabidopsis thaliana</i>, and tomato were clustered into five subfamilies. Gene structure, conserved domain, and motif analyses revealed diverse exon–intron and motif organizations of <i>PtrPUB</i> genes, suggesting potential functional differentiation among <i>PtrPUBs</i>. Cis-acting analysis indicated that the promoters of <i>PtrPUB</i> genes harbor elements related to hormone signaling (ABA, MeJA), drought stress, and low-temperature responses. Transcriptomic data and qRT-PCR results suggested that <i>PtrPUB</i> genes are responsive to ABA and dehydration treatments. This study provides a foundation for understanding the functional roles of <i>PUB</i> genes in trifoliate orange and offers insights for improving stress tolerance in citrus breeding programs.
ISSN:2311-7524

Similar Items