Effects of <sup>60</sup>Co-γ rays radiation on plantlet growth and its cytological change of oriental lily in vitro
The oriental lily scales were irradiated with <sup>60</sup>Co-γ rays and cultured in vitro to induce bud and proliferate. The proliferation buds were induced to root and explore the effect of <sup>60</sup>Co-γ rays radiation on the plantlet growth and its cytological effect....
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.010 |
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| Summary: | The oriental lily scales were irradiated with <sup>60</sup>Co-γ rays and cultured in vitro to induce bud and proliferate. The proliferation buds were induced to root and explore the effect of <sup>60</sup>Co-γ rays radiation on the plantlet growth and its cytological effect. The result shows that the effect of irradiation on the rooting rate was not significant, while its effects on the growth indexes of plantlets, such as number of leaves, number of roots, the root length were most significant. Within the treatments of 0.5-4.0 Gy radiation doses, the height of regenerating plant was declined with the dose increasing. The effect of irradiation on the karyokinesis of root tip cells of plantlets represented stimulating within the treatments of 0.5-1.0 Gy radiation doses and restraining within the treatments of 2.0-4.0 Gy. The cell aberration rate of all the irradiation treatments were higher than the control, the aberration rate of the 2.0 Gy treatment was the highest. Types of chromosome abnormity of root tip cells were affected incoordinately by different doses of irradiation. The effect of irradiation on percent of chromosomal bridge was the most sensitive, then the percent of lag chromosome and chromosomal fragment, the percent of micronuclei aberration was the least sensitive. Chromosomal aberrance types of morphological abnormal shoots were different.1.0 Gy was a fitting dose for the irradiation treatment combining with culture in vitro on the oriental lily scales. |
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| ISSN: | 1008-9209 2097-5155 |