Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle
This study investigates the transcriptome-level alterations that influence production traits and early fattening stage myogenesis in Hanwoo cattle, specifically focusing on the highly prized Longissimus dorsi (LD) and Psoas major (PM) skeletal muscles, which hold significant commercial value. We con...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2024.2379883 |
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| author | Sunirmal Sheet Sun Sik Jang Jin-A. Lim Woncheoul Park Dahye Kim |
| author_facet | Sunirmal Sheet Sun Sik Jang Jin-A. Lim Woncheoul Park Dahye Kim |
| author_sort | Sunirmal Sheet |
| collection | DOAJ |
| description | This study investigates the transcriptome-level alterations that influence production traits and early fattening stage myogenesis in Hanwoo cattle, specifically focusing on the highly prized Longissimus dorsi (LD) and Psoas major (PM) skeletal muscles, which hold significant commercial value. We conducted RNA sequencing analysis on LD and PM muscles from 14 Hanwoo steers (n = 7, each group) at the age of 10 months, all fed the same diet. Our results unveiled a total of 374 and 206 up-regulated differentially expressed genes (DEGs) in LD and PM muscles, respectively, with statistical significance (p < 0.05) and a log2fold change ≥ 1. Genes governing muscle development processes, such as PAX3, MYL3, COL11A1, and MYL6B, were found to be expressed at higher levels in both tissues. Conversely, genes regulating lipid metabolism, including FABP3, FABP4, LEP, ADIPOQ, and PLIN1, exhibited higher expression in the PM muscle. Functional enrichment analysis revealed a tissue-specific response, as PM muscle showed increased lipid metabolism allied pathways, including the PPAR signaling pathway and regulation of lipolysis in adipocytes, while LD was characterized by growth and proliferative processes. Our findings validate the presence of a muscle-dependent transcription and co-expression pattern that elucidates the transcriptional landscape of bovine skeletal muscle. |
| format | Article |
| id | doaj-art-885a2cdf078045babfa571d441c1a063 |
| institution | Matheson Library |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-885a2cdf078045babfa571d441c1a0632025-07-23T08:24:04ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2379883Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattleSunirmal Sheet0Sun Sik Jang1Jin-A. Lim2Woncheoul Park3Dahye Kim4Animal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, South KoreaHanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang, South KoreaAnimal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, South KoreaAnimal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, South KoreaAnimal Genomics and Bioinformatics Division, National Institute of Animal Science, Rural Development Administration, Wanju, South KoreaThis study investigates the transcriptome-level alterations that influence production traits and early fattening stage myogenesis in Hanwoo cattle, specifically focusing on the highly prized Longissimus dorsi (LD) and Psoas major (PM) skeletal muscles, which hold significant commercial value. We conducted RNA sequencing analysis on LD and PM muscles from 14 Hanwoo steers (n = 7, each group) at the age of 10 months, all fed the same diet. Our results unveiled a total of 374 and 206 up-regulated differentially expressed genes (DEGs) in LD and PM muscles, respectively, with statistical significance (p < 0.05) and a log2fold change ≥ 1. Genes governing muscle development processes, such as PAX3, MYL3, COL11A1, and MYL6B, were found to be expressed at higher levels in both tissues. Conversely, genes regulating lipid metabolism, including FABP3, FABP4, LEP, ADIPOQ, and PLIN1, exhibited higher expression in the PM muscle. Functional enrichment analysis revealed a tissue-specific response, as PM muscle showed increased lipid metabolism allied pathways, including the PPAR signaling pathway and regulation of lipolysis in adipocytes, while LD was characterized by growth and proliferative processes. Our findings validate the presence of a muscle-dependent transcription and co-expression pattern that elucidates the transcriptional landscape of bovine skeletal muscle.https://www.tandfonline.com/doi/10.1080/10495398.2024.2379883Hanwoo cattleskeletal muscle transcriptomemyogenesisgene co-expressionlongissimus dorsipsoas major |
| spellingShingle | Sunirmal Sheet Sun Sik Jang Jin-A. Lim Woncheoul Park Dahye Kim Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle Animal Biotechnology Hanwoo cattle skeletal muscle transcriptome myogenesis gene co-expression longissimus dorsi psoas major |
| title | Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle |
| title_full | Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle |
| title_fullStr | Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle |
| title_full_unstemmed | Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle |
| title_short | Molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in Hanwoo cattle |
| title_sort | molecular signatures diversity unveiled through a comparative transcriptome analysis of longissimus dorsi and psoas major muscles in hanwoo cattle |
| topic | Hanwoo cattle skeletal muscle transcriptome myogenesis gene co-expression longissimus dorsi psoas major |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2024.2379883 |
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