Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1
Exchange of antimicrobial resistance genes via mobile genetic elements occur in the gut which can be transferred from mother to neonate during birth. This study is the first to analyse transmissible colistin resistance gene, mcr, in pregnant mothers and neonates. Samples were collected from pregnant...
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2278899 |
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| author | Sharmi Naha Priyanka Basak Kirsty Sands Rebecca Milton Maria J. Carvalho Shravani Mitra Amrita Bhattacharjee Anuradha Sinha Suchandra Mukherjee Bijan Saha Pinaki Chattopadhyay Partha Sarathi Chakravorty Ranjan Kumar Nandy Shanta Dutta Timothy R. Walsh Sulagna Basu |
| author_facet | Sharmi Naha Priyanka Basak Kirsty Sands Rebecca Milton Maria J. Carvalho Shravani Mitra Amrita Bhattacharjee Anuradha Sinha Suchandra Mukherjee Bijan Saha Pinaki Chattopadhyay Partha Sarathi Chakravorty Ranjan Kumar Nandy Shanta Dutta Timothy R. Walsh Sulagna Basu |
| author_sort | Sharmi Naha |
| collection | DOAJ |
| description | Exchange of antimicrobial resistance genes via mobile genetic elements occur in the gut which can be transferred from mother to neonate during birth. This study is the first to analyse transmissible colistin resistance gene, mcr, in pregnant mothers and neonates. Samples were collected from pregnant mothers (rectal) and septicaemic neonates (rectal and blood) and analysed for the presence of mcr, its transmissibility, genome diversity, and exchange of mcr between isolates within an individual and across different individuals (not necessarily mother–baby pairs). mcr-1.1 was detected in rectal samples of pregnant mothers (n = 10, 0.9%), but not in neonates. All mcr-positive mothers gave birth to healthy neonates from whom rectal specimen were not collected. Hence, the transmission of mcr between these mother-neonate pairs could not be studied. mcr-1.1 was noted only in Escherichia coli (phylogroup A & B1), and carried few resistance and virulence genes. Isolates belonged to diverse sequence types (n = 11) with two novel STs (ST12452, ST12455). mcr-1.1 was borne on conjugative IncHI2 bracketed between ISApl1 on Tn6630, and the plasmids exhibited similarities in sequences across the study isolates. Phylogenetic comparison showed that study isolates were related to mcr-positive isolates of animal origin from Southeast Asian countries. Spread of mcr-1.1 within this study occurred either via similar mcr-positive clones or similar mcr-bearing plasmids in mothers. Though this study could not build evidence for mother–baby transmission but the presence of such genes in the maternal specimen may enhance the chances of transmission to neonates. |
| format | Article |
| id | doaj-art-86ff746e48e14f7e8303f4309e0848a3 |
| institution | Matheson Library |
| issn | 2222-1751 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
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| series | Emerging Microbes and Infections |
| spelling | doaj-art-86ff746e48e14f7e8303f4309e0848a32025-07-02T17:44:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112210.1080/22221751.2023.2278899Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1Sharmi Naha0Priyanka Basak1Kirsty Sands2Rebecca Milton3Maria J. Carvalho4Shravani Mitra5Amrita Bhattacharjee6Anuradha Sinha7Suchandra Mukherjee8Bijan Saha9Pinaki Chattopadhyay10Partha Sarathi Chakravorty11Ranjan Kumar Nandy12Shanta Dutta13Timothy R. Walsh14Sulagna Basu15Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaInstitute of Infection and Immunity, Cardiff University, Cardiff, UKInstitute of Infection and Immunity, Cardiff University, Cardiff, UKInstitute of Infection and Immunity, Cardiff University, Cardiff, UKDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaDepartment of Neonatology, Institute of Post-Graduate and Medical Education & Research, Kolkata, IndiaDepartment of Neonatology, Institute of Post-Graduate and Medical Education & Research, Kolkata, IndiaDepartment of Neonatology, Institute of Post-Graduate and Medical Education & Research, Kolkata, IndiaDepartment of Obstetrics & Gynecology, Institute of Post-Graduate and Medical Education & Research, Kolkata, IndiaDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaInstitute of Infection and Immunity, Cardiff University, Cardiff, UKDivision of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, IndiaExchange of antimicrobial resistance genes via mobile genetic elements occur in the gut which can be transferred from mother to neonate during birth. This study is the first to analyse transmissible colistin resistance gene, mcr, in pregnant mothers and neonates. Samples were collected from pregnant mothers (rectal) and septicaemic neonates (rectal and blood) and analysed for the presence of mcr, its transmissibility, genome diversity, and exchange of mcr between isolates within an individual and across different individuals (not necessarily mother–baby pairs). mcr-1.1 was detected in rectal samples of pregnant mothers (n = 10, 0.9%), but not in neonates. All mcr-positive mothers gave birth to healthy neonates from whom rectal specimen were not collected. Hence, the transmission of mcr between these mother-neonate pairs could not be studied. mcr-1.1 was noted only in Escherichia coli (phylogroup A & B1), and carried few resistance and virulence genes. Isolates belonged to diverse sequence types (n = 11) with two novel STs (ST12452, ST12455). mcr-1.1 was borne on conjugative IncHI2 bracketed between ISApl1 on Tn6630, and the plasmids exhibited similarities in sequences across the study isolates. Phylogenetic comparison showed that study isolates were related to mcr-positive isolates of animal origin from Southeast Asian countries. Spread of mcr-1.1 within this study occurred either via similar mcr-positive clones or similar mcr-bearing plasmids in mothers. Though this study could not build evidence for mother–baby transmission but the presence of such genes in the maternal specimen may enhance the chances of transmission to neonates.https://www.tandfonline.com/doi/10.1080/22221751.2023.2278899Colistin-resistant Escherichia colipregnant mother and neonatal gut carriagemcr-1.1-bearing IncHI2transmission dynamics of mcr-1.1Illumina & MinION nanopore sequencing |
| spellingShingle | Sharmi Naha Priyanka Basak Kirsty Sands Rebecca Milton Maria J. Carvalho Shravani Mitra Amrita Bhattacharjee Anuradha Sinha Suchandra Mukherjee Bijan Saha Pinaki Chattopadhyay Partha Sarathi Chakravorty Ranjan Kumar Nandy Shanta Dutta Timothy R. Walsh Sulagna Basu Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 Emerging Microbes and Infections Colistin-resistant Escherichia coli pregnant mother and neonatal gut carriage mcr-1.1-bearing IncHI2 transmission dynamics of mcr-1.1 Illumina & MinION nanopore sequencing |
| title | Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 |
| title_full | Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 |
| title_fullStr | Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 |
| title_full_unstemmed | Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 |
| title_short | Carriage and within-host diversity of mcr-1.1-harbouring Escherichia coli from pregnant mothers: inter- and intra-mother transmission dynamics of mcr-1.1 |
| title_sort | carriage and within host diversity of mcr 1 1 harbouring escherichia coli from pregnant mothers inter and intra mother transmission dynamics of mcr 1 1 |
| topic | Colistin-resistant Escherichia coli pregnant mother and neonatal gut carriage mcr-1.1-bearing IncHI2 transmission dynamics of mcr-1.1 Illumina & MinION nanopore sequencing |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2278899 |
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