Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability
The low egg production of goose greatly limits the development of the industry. China possesses the most abundant goose breeds resources. In this study, genome resequencing data of swan goose (Anser cygnoides) and domesticated high and low laying goose breeds (Anser cygnoides domestiation) were used...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923001533 |
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| author | Qingyuan Ouyang Hengli Xie Shenqiang Hu Cong Lan Mingxia Ran Jiwei Hu Hua He Liang Li Hehe Liu Hao Qu Jiwen Wang |
| author_facet | Qingyuan Ouyang Hengli Xie Shenqiang Hu Cong Lan Mingxia Ran Jiwei Hu Hua He Liang Li Hehe Liu Hao Qu Jiwen Wang |
| author_sort | Qingyuan Ouyang |
| collection | DOAJ |
| description | The low egg production of goose greatly limits the development of the industry. China possesses the most abundant goose breeds resources. In this study, genome resequencing data of swan goose (Anser cygnoides) and domesticated high and low laying goose breeds (Anser cygnoides domestiation) were used to identify key genes related to egg laying ability in geese and verify their functions. Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese. Furthermore, SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese. The results showed that RTCB, BPIFC, SYN3, SYNE1, VIP, and ESR1 may be related to the differences in laying ability of geese. Notably, only ESR1 was identified simultaneously by GWAS and selective sweep analysis. The genotype of Indelchr3:54429172, located downstream of ESR1, was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese. CCK-8, EdU, and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells (phGCs) and inhibit their proliferation. Combined with transcriptome data, it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways. Overall, our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability. The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods. These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese. |
| format | Article |
| id | doaj-art-861aaf9e028b46ae8ac8c76720c9c94e |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-861aaf9e028b46ae8ac8c76720c9c94e2025-08-02T12:49:50ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192025-03-0124311671180Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying abilityQingyuan Ouyang0Hengli Xie1Shenqiang Hu2Cong Lan3Mingxia Ran4Jiwei Hu5Hua He6Liang Li7Hehe Liu8Hao Qu9Jiwen Wang10Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaState Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; State Key Laboratory of Swine and Poultry Breeding Industry, Chengdu 611830, China; Correspondence Jiwen Wang, Tel: +86-28-86291010The low egg production of goose greatly limits the development of the industry. China possesses the most abundant goose breeds resources. In this study, genome resequencing data of swan goose (Anser cygnoides) and domesticated high and low laying goose breeds (Anser cygnoides domestiation) were used to identify key genes related to egg laying ability in geese and verify their functions. Selective sweep analyses revealed 416 genes that were specifically selected during the domestication process from swan geese to high laying geese. Furthermore, SNPs and Indels markers were used in GWAS analyses between high and low laying breed geese. The results showed that RTCB, BPIFC, SYN3, SYNE1, VIP, and ESR1 may be related to the differences in laying ability of geese. Notably, only ESR1 was identified simultaneously by GWAS and selective sweep analysis. The genotype of Indelchr3:54429172, located downstream of ESR1, was confirmed to affect the expression of ESR1 in the ovarian stroma and showed significant correlation with body weight at first egg and laying frequency of geese. CCK-8, EdU, and flow cytometry confirmed that ESR1 can promote the apoptosis of goose pre-hierarchical follicles ganulosa cells (phGCs) and inhibit their proliferation. Combined with transcriptome data, it was found ESR1 involved in the function of goose phGCs may be related to MAPK and TGF-beta signaling pathways. Overall, our study used genomic information from different goose breeds to identify an indel located in the downstream of ESR1 associated with goose laying ability. The main pathways and biological processes of ESR1 involved in the regulation of goose laying ability were identified by cell biology and transcriptomics methods. These results are helpful to further understand the laying ability characteristics of goose and improve the egg production of geese.http://www.sciencedirect.com/science/article/pii/S2095311923001533gooselaying abilitygenomeESR1 |
| spellingShingle | Qingyuan Ouyang Hengli Xie Shenqiang Hu Cong Lan Mingxia Ran Jiwei Hu Hua He Liang Li Hehe Liu Hao Qu Jiwen Wang Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability Journal of Integrative Agriculture goose laying ability genome ESR1 |
| title | Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability |
| title_full | Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability |
| title_fullStr | Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability |
| title_full_unstemmed | Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability |
| title_short | Comparative genomics study between high and low laying goose breeds reveals the important role of ESR1 in laying ability |
| title_sort | comparative genomics study between high and low laying goose breeds reveals the important role of esr1 in laying ability |
| topic | goose laying ability genome ESR1 |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923001533 |
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