Molecular Diagnosis of Scrub Typhus: Sample and Timing Matter
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Compuscript Ltd
2024-01-01
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| Series: | Zoonoses |
| Online Access: | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2023-0019 |
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| Summary: | <div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section">
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<h5 class="section-title" id="d1446636e157">Objective:</h5>
<p dir="auto" id="d1446636e159">Scrub typhus (ST) is an acute febrile illness caused by
<i>Orientia tsutsugamushi</i>. Laboratory tests are needed to confirm the diagnosis when the characteristic eschar
is absent. This study aimed to evaluate the performance of 47 kDa qPCR using clotted
and EDTA-treated blood among patients with suspected ST
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<h5 class="section-title" id="d1446636e165">Methods:</h5>
<p dir="auto" id="d1446636e167">Clotted blood samples from 284 inpatients (IPs) and EDTA-treated blood samples from
194 outpatients (OPs) with suspected scrub typhus were collected from patients who
were blood culture- and malaria-negative. ST IgM ELISA and 47 kDa qPCR were performed
for detection of ST infections.
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<h5 class="section-title" id="d1446636e170">Results:</h5>
<p dir="auto" id="d1446636e172">Among the IPs (n=284) and OPs (n=194), 41% and 63% were confirmed to have ST based
on the case definition, respectively; the mortality rate was 1%. The 47 kDa qPCR sensitivity
of the buffy coat (65.6%) was greater compared to clotted blood (37.1%). PCR was more
likely to be positive in the 1st week of the illness; IgM ELISA positivity increased
after the 5th day of the illness.
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<h5 class="section-title" id="d1446636e175">Conclusion:</h5>
<p dir="auto" id="d1446636e177">The type of sample and the time of sample collection have a role in the diagnosis
of ST. These preliminary results need to be confirmed by prospective multicentric
studies.
</p>
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| ISSN: | 2737-7466 2737-7474 |