Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2
Abstract Tiller number is a crucial determinant of grain yield in wheat (Triticum aestivum L.), and identifying functional alleles can enhance our understanding of wheat tiller development. Here, we describe the ot2 wheat mutant, which exhibits a 91% reduction in tiller number compared to the wild t...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.70043 |
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| author | Chenxi Wang Jiaxing Bai Hongchun Xiong Yongdun Xie Jiayu Gu Linshu Zhao Huiyuan Li Yuping Ding Xinmei Guo Huijun Guo Luxiang Liu |
| author_facet | Chenxi Wang Jiaxing Bai Hongchun Xiong Yongdun Xie Jiayu Gu Linshu Zhao Huiyuan Li Yuping Ding Xinmei Guo Huijun Guo Luxiang Liu |
| author_sort | Chenxi Wang |
| collection | DOAJ |
| description | Abstract Tiller number is a crucial determinant of grain yield in wheat (Triticum aestivum L.), and identifying functional alleles can enhance our understanding of wheat tiller development. Here, we describe the ot2 wheat mutant, which exhibits a 91% reduction in tiller number compared to the wild type and displays inhibited tiller bud differentiation from the 2–3 leaf stage. Bulked segregant analysis combined with exon sequencing mapped the Taot2 gene to chromosome 1BL. Fourteen kompetitive allele‐specific PCR markers were developed and utilized to narrow down the region containing Taot2 to a 2.22 Mb interval. This region encompasses 39 high‐confidence genes. Through transcriptomic analysis and functional gene investigation, TraesCS1B03G1126300 was identified as the candidate gene, encoding an auxin‐responsive protein of the auxin/indole‐3‐acetic acid family. Our findings provide the foundation for map‐based cloning of Taot2, a novel wheat tiller gene, and offer valuable insights into wheat tiller bud initiation. |
| format | Article |
| id | doaj-art-83e223d95bbd4cd09d3033d35d76f71e |
| institution | Matheson Library |
| issn | 1940-3372 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-83e223d95bbd4cd09d3033d35d76f71e2025-06-27T07:10:58ZengWileyThe Plant Genome1940-33722025-06-01182n/an/a10.1002/tpg2.70043Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2Chenxi Wang0Jiaxing Bai1Hongchun Xiong2Yongdun Xie3Jiayu Gu4Linshu Zhao5Huiyuan Li6Yuping Ding7Xinmei Guo8Huijun Guo9Luxiang Liu10College of Life Sciences, Key Laboratory of Major Crop Germplasm Innovation and Application in Qingdao Qingdao Agricultural University Qingdao ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaCollege of Life Sciences, Key Laboratory of Major Crop Germplasm Innovation and Application in Qingdao Qingdao Agricultural University Qingdao ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaState Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing ChinaAbstract Tiller number is a crucial determinant of grain yield in wheat (Triticum aestivum L.), and identifying functional alleles can enhance our understanding of wheat tiller development. Here, we describe the ot2 wheat mutant, which exhibits a 91% reduction in tiller number compared to the wild type and displays inhibited tiller bud differentiation from the 2–3 leaf stage. Bulked segregant analysis combined with exon sequencing mapped the Taot2 gene to chromosome 1BL. Fourteen kompetitive allele‐specific PCR markers were developed and utilized to narrow down the region containing Taot2 to a 2.22 Mb interval. This region encompasses 39 high‐confidence genes. Through transcriptomic analysis and functional gene investigation, TraesCS1B03G1126300 was identified as the candidate gene, encoding an auxin‐responsive protein of the auxin/indole‐3‐acetic acid family. Our findings provide the foundation for map‐based cloning of Taot2, a novel wheat tiller gene, and offer valuable insights into wheat tiller bud initiation.https://doi.org/10.1002/tpg2.70043 |
| spellingShingle | Chenxi Wang Jiaxing Bai Hongchun Xiong Yongdun Xie Jiayu Gu Linshu Zhao Huiyuan Li Yuping Ding Xinmei Guo Huijun Guo Luxiang Liu Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 The Plant Genome |
| title | Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 |
| title_full | Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 |
| title_fullStr | Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 |
| title_full_unstemmed | Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 |
| title_short | Characterization of wheat oligo‐tiller mutant ot2 and fine mapping of the mutant gene Taot2 |
| title_sort | characterization of wheat oligo tiller mutant ot2 and fine mapping of the mutant gene taot2 |
| url | https://doi.org/10.1002/tpg2.70043 |
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