3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold
We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Budapest University of Technology and Economics
2019-07-01
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| Series: | eXPRESS Polymer Letters |
| Subjects: | |
| Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0009811&mi=cd |
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| author | A. E. Efimov O. I. Agapova L. A. Safonova M. M. Bobrova V. A. Parfenov E. V. Koudan F. D. A. S. Pereira E. A. Bulanova V. A. Mironov I. I. Agapov |
| author_facet | A. E. Efimov O. I. Agapova L. A. Safonova M. M. Bobrova V. A. Parfenov E. V. Koudan F. D. A. S. Pereira E. A. Bulanova V. A. Mironov I. I. Agapov |
| author_sort | A. E. Efimov |
| collection | DOAJ |
| description | We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale. |
| format | Article |
| id | doaj-art-839f37cd6dd34c86aa2bc42bd206868f |
| institution | Matheson Library |
| issn | 1788-618X |
| language | English |
| publishDate | 2019-07-01 |
| publisher | Budapest University of Technology and Economics |
| record_format | Article |
| series | eXPRESS Polymer Letters |
| spelling | doaj-art-839f37cd6dd34c86aa2bc42bd206868f2025-08-02T12:36:22ZengBudapest University of Technology and EconomicseXPRESS Polymer Letters1788-618X2019-07-0113763264110.3144/expresspolymlett.2019.533D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffoldA. E. EfimovO. I. AgapovaL. A. SafonovaM. M. BobrovaV. A. ParfenovE. V. KoudanF. D. A. S. PereiraE. A. BulanovaV. A. MironovI. I. AgapovWe present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale.http://www.expresspolymlett.com/letolt.php?file=EPL-0009811&mi=cdBiocompatible polymersElectrospinningNanotomographyTissue spheroidsBioprinting |
| spellingShingle | A. E. Efimov O. I. Agapova L. A. Safonova M. M. Bobrova V. A. Parfenov E. V. Koudan F. D. A. S. Pereira E. A. Bulanova V. A. Mironov I. I. Agapov 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold eXPRESS Polymer Letters Biocompatible polymers Electrospinning Nanotomography Tissue spheroids Bioprinting |
| title | 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| title_full | 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| title_fullStr | 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| title_full_unstemmed | 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| title_short | 3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| title_sort | 3d scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold |
| topic | Biocompatible polymers Electrospinning Nanotomography Tissue spheroids Bioprinting |
| url | http://www.expresspolymlett.com/letolt.php?file=EPL-0009811&mi=cd |
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