3D scanning probe nanotomography of tissue spheroid fibroblasts interacting with electrospun polyurethane scaffold
We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers...
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Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Budapest University of Technology and Economics
2019-07-01
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Series: | eXPRESS Polymer Letters |
Subjects: | |
Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0009811&mi=cd |
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Summary: | We present a 3D study of nanostructural features of a bioprinted tissue spheroid interacting with polyurethane dual-scale biocompatible scaffold manufactured by three-dimensional printing and electrospinning. Three-dimensional analysis of fibroblasts interacting with electrospun polyurethane fibers was conducted using scanning probe nanotomography with an experimental setup combining ultramicrotome and a scanning probe microscope. Three-dimensional reconstruction demonstrates direct visualization of cell membrane protrusions and coherent cell-fiber interfaces, the formation of which is a prerequisite for an efficient tissue engineered implant. Analysis of obtained 3D data allows for quantitative calculation of the important morphological parameters of adhered cells, scaffolds, and cell-scaffold interfaces. The proposed method may be successfully applied to investigate 3D cell-scaffold constructs at nanoscale. |
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ISSN: | 1788-618X |