Synthesis and identification of artificial antigen for chloramphenicol
In order to establish the immunoassay method of chloramphenicol (CAP), two haptens (CAP-HS<sub>1</sub>, CAP-HS2) were synthesized by chloramphenicol and succinic anhydride and identified by ESI-MS. The haptens were conjugated to bovine serum albumin (BSA), with the modified active ester...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2006-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2006.02.0180 |
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| Summary: | In order to establish the immunoassay method of chloramphenicol (CAP), two haptens (CAP-HS<sub>1</sub>, CAP-HS2) were synthesized by chloramphenicol and succinic anhydride and identified by ESI-MS. The haptens were conjugated to bovine serum albumin (BSA), with the modified active ester method and used as immuneogens to produce their corresponding antibodies in rabbits. Titers of antibodies against CAP-HS<sub>1</sub> and CAP-HS<sub>2</sub> were 8.39 × 10<sup>6</sup> and 6.55 × 10<sup>4</sup>, respectively, at the concentration of coating antigens 10 μg·mL<sup>-1</sup>. Based on the affinity and specificity of the antibodies, antibody against CAP-HS<sub>1</sub> was adopted in the immunoassay. The lineal range of antibody against CAP-HS<sub>1</sub> reacting with chloramphenicol was from 0.2 to 100 ng·mL<sup>-1</sup>, the limit of detection (LOD) being 0.239 ng·mL<sup>-1</sup>. The percentages of cross-activity to thiamphenicol, florfenicol, and other antibiotics were 0.025%, 0.166%, and less than 2×10<sup>-5</sup>%, respectively, in this study. |
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| ISSN: | 1008-9209 2097-5155 |