Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis
MicroRNAs (miRNAs) have been implicated in a variety of disorders. Although studies have examined miRNA in pediatric atopic dermatitis (AD), the impact of topical corticosteroid (TCS) therapy on miRNA expression in pediatric AD has not been investigated. We sought to investigate the effects of 6 wee...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | JID Innovations |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266702672500044X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839639836173008896 |
|---|---|
| author | Janna Nousbeck Maeve A. McAleer Elaine M. Kenny Alan D. Irvine |
| author_facet | Janna Nousbeck Maeve A. McAleer Elaine M. Kenny Alan D. Irvine |
| author_sort | Janna Nousbeck |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) have been implicated in a variety of disorders. Although studies have examined miRNA in pediatric atopic dermatitis (AD), the impact of topical corticosteroid (TCS) therapy on miRNA expression in pediatric AD has not been investigated. We sought to investigate the effects of 6 weeks of TCS therapy on miRNA expression in infants with AD. Small RNA sequencing and real-time RT-qPCR were performed to identify differentially expressed miRNAs in PBMCs of infants with AD after TCS treatment; HTG EdgeSeq was used to identify differentially expressed miRNAs in plasma. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was conducted using a list of experimentally verified miRNA targets sourced from the DIANA-TarBase and miRTarBase databases. Five miRNAs were differentially expressed in circulating PBMCs after TCS treatment (miR-143-3p, miR-27a-5p, miR-126-3p, miR-451a, and miR-223-3p); 12 miRNAs were differentially expressed in plasma. These miRNAs have regulatory functions crucial for regulating cell growth and survival, vascular adhesion, angiogenesis, skin barrier integrity, stress and nervous system processes, immune responses, inflammation, and T helper 17 cell differentiation. TCS treatment led to a distinct miRNA expression profile in peripheral blood, providing insights into how this treatment impacts disease mechanisms in childhood AD. |
| format | Article |
| id | doaj-art-81b1c6cd6aad48f8987c8dc2df14d2d6 |
| institution | Matheson Library |
| issn | 2667-0267 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JID Innovations |
| spelling | doaj-art-81b1c6cd6aad48f8987c8dc2df14d2d62025-07-04T04:47:14ZengElsevierJID Innovations2667-02672025-09-0155100388Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic DermatitisJanna Nousbeck0Maeve A. McAleer1Elaine M. Kenny2Alan D. Irvine3Clinical Medicine, Trinity College Dublin, Dublin, IrelandClinical Medicine, Trinity College Dublin, Dublin, Ireland; Paediatric Dermatology, Children’s Health Ireland at Crumlin, Dublin, IrelandTrinSeq, Department of Psychiatry, Trinity Translational Medicine Institute, Trinity College Dublin, St James’s Hospital, Dublin, IrelandClinical Medicine, Trinity College Dublin, Dublin, Ireland; Paediatric Dermatology, Children’s Health Ireland at Crumlin, Dublin, Ireland; Correspondence: Alan D. Irvine, Clinical Medicine, Trinity College Dublin, Dublin B12, Ireland.MicroRNAs (miRNAs) have been implicated in a variety of disorders. Although studies have examined miRNA in pediatric atopic dermatitis (AD), the impact of topical corticosteroid (TCS) therapy on miRNA expression in pediatric AD has not been investigated. We sought to investigate the effects of 6 weeks of TCS therapy on miRNA expression in infants with AD. Small RNA sequencing and real-time RT-qPCR were performed to identify differentially expressed miRNAs in PBMCs of infants with AD after TCS treatment; HTG EdgeSeq was used to identify differentially expressed miRNAs in plasma. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was conducted using a list of experimentally verified miRNA targets sourced from the DIANA-TarBase and miRTarBase databases. Five miRNAs were differentially expressed in circulating PBMCs after TCS treatment (miR-143-3p, miR-27a-5p, miR-126-3p, miR-451a, and miR-223-3p); 12 miRNAs were differentially expressed in plasma. These miRNAs have regulatory functions crucial for regulating cell growth and survival, vascular adhesion, angiogenesis, skin barrier integrity, stress and nervous system processes, immune responses, inflammation, and T helper 17 cell differentiation. TCS treatment led to a distinct miRNA expression profile in peripheral blood, providing insights into how this treatment impacts disease mechanisms in childhood AD.http://www.sciencedirect.com/science/article/pii/S266702672500044XAtopic dermatitisBiomarkersCorticosteroidsmiRNATherapeutics |
| spellingShingle | Janna Nousbeck Maeve A. McAleer Elaine M. Kenny Alan D. Irvine Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis JID Innovations Atopic dermatitis Biomarkers Corticosteroids miRNA Therapeutics |
| title | Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis |
| title_full | Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis |
| title_fullStr | Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis |
| title_full_unstemmed | Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis |
| title_short | Effect of Topical Corticosteroid Treatment on microRNA Expression in Infants with Atopic Dermatitis |
| title_sort | effect of topical corticosteroid treatment on microrna expression in infants with atopic dermatitis |
| topic | Atopic dermatitis Biomarkers Corticosteroids miRNA Therapeutics |
| url | http://www.sciencedirect.com/science/article/pii/S266702672500044X |
| work_keys_str_mv | AT jannanousbeck effectoftopicalcorticosteroidtreatmentonmicrornaexpressionininfantswithatopicdermatitis AT maeveamcaleer effectoftopicalcorticosteroidtreatmentonmicrornaexpressionininfantswithatopicdermatitis AT elainemkenny effectoftopicalcorticosteroidtreatmentonmicrornaexpressionininfantswithatopicdermatitis AT alandirvine effectoftopicalcorticosteroidtreatmentonmicrornaexpressionininfantswithatopicdermatitis |