The antiviral activity of cerium and lanthanum nanooxides modified with silver
Today, the antiviral activity of oxide nanomaterials can be used in the fight against the viral disease COVID-19. It is thought that Ag nanoparticles may bind to the surface glycoprotein of the virus and interfere with the virus’s interaction with epithelial cells, and inhibit virus reproduction by...
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Chuiko Institute of Surface Chemistry of NAS of Ukraine
2023-05-01
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| Series: | Хімія, фізика та технологія поверхні |
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| Online Access: | https://cpts.com.ua/index.php/cpts/article/view/679 |
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| author | M. M. Zahornyi O. M. Lavrynenko O. Yu Pavlenko O. Yu Povnitsa L. O. Artiukh K. S. Naumenko S. D. Zahorodnia A. I. Ievtushenko |
| author_facet | M. M. Zahornyi O. M. Lavrynenko O. Yu Pavlenko O. Yu Povnitsa L. O. Artiukh K. S. Naumenko S. D. Zahorodnia A. I. Ievtushenko |
| author_sort | M. M. Zahornyi |
| collection | DOAJ |
| description |
Today, the antiviral activity of oxide nanomaterials can be used in the fight against the viral disease COVID-19. It is thought that Ag nanoparticles may bind to the surface glycoprotein of the virus and interfere with the virus’s interaction with epithelial cells, and inhibit virus reproduction by releasing silver ions in the cell. The viruses’ inhibition with RNA (ribonucleic acid) and DNA (deoxyribonucleic acid) genomes by oxide nanocomposites action was presented.
In this research, the surface structure of doped CeO2 (La2O3) was studied by nitrogen adsorption-desorption based on BET method. The silver atom’s existence in CeO2 - Ag0 can facilitate the transport of more holes to the surface and can enhance the optical, antivirus activity. The primary particle size of pure cerium dioxide is 7 nm, for CeO2 - Ag composite at 2 and 4 wt. % of silver is 6.5 and 6.9 nm; for La2O3 - Ag 27 and 35 nm, respectively.
Cell viability was assessed using an MTT (3-(4,5-Dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide) assay after NPs (nanoparticles) exposure, since only viable cells have functional mitochondrial dehydrogenase enzymes that can reduce MTT to formazan. Nanoparticles were non-toxic for BHK-21(Syrian hamster kidney), Hep-2 (Human larynx carcinoma), and MDCK (Canine kidney) cells in concentrations of 10 and 100 ?g/ml, while cell viability was within 76?100 %. La2O3 and CeO2, which contained 4 wt. % of Ag at a concentration of 1000 ?g/ml had a lower toxic effect: for BHK-21 cells 68 and 76 % of viable cells, respectively; for Hep-2 - 40 and 36 %, for MDCK - 42 and 48 %; La2O3 and CeO2 with 2 and 5 wt. % of Ag at a concentration of 1000 µg/ml were highly toxic. The level of ВНК-21, Нер-2, and MDCK cells viability was in a range of 7 to 37 %.
It has been stated that oxides of cerium and lanthanum have a pronounced virucidal action against the Herpes simplex virus and Influenza A virus by completely inhibiting the development of its cytopathic action. The lanthanum and cerium oxides with 2 and 5 wt. % of silver inhibited the development of the virus’s CPE by more than 5.0 log10 compared to the virus control. The results show that lanthanum and cerium oxides with 2 and 5 wt. % silver have a high virucidal effect against herpes simplex virus type 1. A 1.0?4.0 log10 reduction in the infectious titer of the Herpes virus synthesized “de novo” in the presence of lanthanum and cerium oxide nanocomposites has been shown.
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| format | Article |
| id | doaj-art-802ed92aaf7f47ea9cf7a77dbe7c1ba6 |
| institution | Matheson Library |
| issn | 2079-1704 2518-1238 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Chuiko Institute of Surface Chemistry of NAS of Ukraine |
| record_format | Article |
| series | Хімія, фізика та технологія поверхні |
| spelling | doaj-art-802ed92aaf7f47ea9cf7a77dbe7c1ba62025-07-22T18:24:20ZengChuiko Institute of Surface Chemistry of NAS of UkraineХімія, фізика та технологія поверхні2079-17042518-12382023-05-0114210.15407/hftp14.02.262The antiviral activity of cerium and lanthanum nanooxides modified with silverM. M. Zahornyi0O. M. Lavrynenko1O. Yu Pavlenko2O. Yu Povnitsa3L. O. Artiukh4K. S. Naumenko5S. D. Zahorodnia6A. I. Ievtushenko7Frantsevich Institute for Problems of Materials Science of National Academy of Sciences of UkraineFrantsevich Institute for Problems of Materials Science of National Academy of Sciences of UkraineFrantsevich Institute for Problems of Materials Science of National Academy of Sciences of UkraineD.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of UkraineD.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of UkraineD.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of UkraineD.K. Zabolotny Institute of Microbiology and Virology of National Academy of Sciences of UkraineFrantsevich Institute for Problems of Materials Science of National Academy of Sciences of Ukraine Today, the antiviral activity of oxide nanomaterials can be used in the fight against the viral disease COVID-19. It is thought that Ag nanoparticles may bind to the surface glycoprotein of the virus and interfere with the virus’s interaction with epithelial cells, and inhibit virus reproduction by releasing silver ions in the cell. The viruses’ inhibition with RNA (ribonucleic acid) and DNA (deoxyribonucleic acid) genomes by oxide nanocomposites action was presented. In this research, the surface structure of doped CeO2 (La2O3) was studied by nitrogen adsorption-desorption based on BET method. The silver atom’s existence in CeO2 - Ag0 can facilitate the transport of more holes to the surface and can enhance the optical, antivirus activity. The primary particle size of pure cerium dioxide is 7 nm, for CeO2 - Ag composite at 2 and 4 wt. % of silver is 6.5 and 6.9 nm; for La2O3 - Ag 27 and 35 nm, respectively. Cell viability was assessed using an MTT (3-(4,5-Dimethylthiazol 2-yl)-2,5-diphenyltetrazolium bromide) assay after NPs (nanoparticles) exposure, since only viable cells have functional mitochondrial dehydrogenase enzymes that can reduce MTT to formazan. Nanoparticles were non-toxic for BHK-21(Syrian hamster kidney), Hep-2 (Human larynx carcinoma), and MDCK (Canine kidney) cells in concentrations of 10 and 100 ?g/ml, while cell viability was within 76?100 %. La2O3 and CeO2, which contained 4 wt. % of Ag at a concentration of 1000 ?g/ml had a lower toxic effect: for BHK-21 cells 68 and 76 % of viable cells, respectively; for Hep-2 - 40 and 36 %, for MDCK - 42 and 48 %; La2O3 and CeO2 with 2 and 5 wt. % of Ag at a concentration of 1000 µg/ml were highly toxic. The level of ВНК-21, Нер-2, and MDCK cells viability was in a range of 7 to 37 %. It has been stated that oxides of cerium and lanthanum have a pronounced virucidal action against the Herpes simplex virus and Influenza A virus by completely inhibiting the development of its cytopathic action. The lanthanum and cerium oxides with 2 and 5 wt. % of silver inhibited the development of the virus’s CPE by more than 5.0 log10 compared to the virus control. The results show that lanthanum and cerium oxides with 2 and 5 wt. % silver have a high virucidal effect against herpes simplex virus type 1. A 1.0?4.0 log10 reduction in the infectious titer of the Herpes virus synthesized “de novo” in the presence of lanthanum and cerium oxide nanocomposites has been shown. https://cpts.com.ua/index.php/cpts/article/view/679cytotoxicityadenovirusHerpes simplex virusInfluenza A virusantiviral activityvirucidal action |
| spellingShingle | M. M. Zahornyi O. M. Lavrynenko O. Yu Pavlenko O. Yu Povnitsa L. O. Artiukh K. S. Naumenko S. D. Zahorodnia A. I. Ievtushenko The antiviral activity of cerium and lanthanum nanooxides modified with silver Хімія, фізика та технологія поверхні cytotoxicity adenovirus Herpes simplex virus Influenza A virus antiviral activity virucidal action |
| title | The antiviral activity of cerium and lanthanum nanooxides modified with silver |
| title_full | The antiviral activity of cerium and lanthanum nanooxides modified with silver |
| title_fullStr | The antiviral activity of cerium and lanthanum nanooxides modified with silver |
| title_full_unstemmed | The antiviral activity of cerium and lanthanum nanooxides modified with silver |
| title_short | The antiviral activity of cerium and lanthanum nanooxides modified with silver |
| title_sort | antiviral activity of cerium and lanthanum nanooxides modified with silver |
| topic | cytotoxicity adenovirus Herpes simplex virus Influenza A virus antiviral activity virucidal action |
| url | https://cpts.com.ua/index.php/cpts/article/view/679 |
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