Assessment of the Adjuvant Effects of Lentinan on the Tuberculosis Subunit Vaccine BG
<b>Objectives</b>: This study aims to assess the adjuvant effects of lentinan and its combination with Mn(J), a manganese-based colloidal adjuvant, on the BG (fusion protein BfrB-GrpE of <i>Mycobacterium tuberculosis</i>) subunit vaccine. <b>Methods</b>: A rabbit...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
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| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/13/6/597 |
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| Summary: | <b>Objectives</b>: This study aims to assess the adjuvant effects of lentinan and its combination with Mn(J), a manganese-based colloidal adjuvant, on the BG (fusion protein BfrB-GrpE of <i>Mycobacterium tuberculosis</i>) subunit vaccine. <b>Methods</b>: A rabbit skin infection model was established to evaluate the immune protection conferred by the BG–lentinan vaccine, the BG–lentinan/Mn(J) vaccine, and the Bacillus Calmette-Guérin (BCG) vaccine against tuberculosis. Rabbits were vaccinated at weeks 0, 2, and 4. Six weeks post-vaccination, antigen-specific IgG levels were measured, followed by a BCG skin challenge. <b>Results</b>: Both the BG–lentinan and BG–lentinan/Mn(J) vaccines significantly increased antigen-specific IgG levels against BfrB and GrpE in rabbits (<i>p</i> < 0.05). Furthermore, these vaccines accelerated the pathological process following BCG infection. The bacterial load in nodules was notably reduced, with the BG–lentinan vaccine group exhibiting the lowest levels (<i>p</i> < 0.01). <b>Conclusions</b>: Lentinan and its combined adjuvant, lentinan/Mn(J), significantly enhance the immune response elicited by the BG tuberculosis subunit vaccine, providing effective protection. |
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| ISSN: | 2076-393X |