Exploring affinity receptors in bioanalysis: from natural binders to biomimetics
Affinity receptors have played a pivotal role in advancing bioanalysis, primarily for diagnostic and therapeutic purposes, thanks to their high selectivity toward target molecules, which enables their use in complex biofluids. While antibodies remain the gold standard, ongoing research has explored...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-12-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053925000773 |
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| author | F. Vivaldi F. Torrini F. Spiaggia F. Di Francesco M. Minunni |
| author_facet | F. Vivaldi F. Torrini F. Spiaggia F. Di Francesco M. Minunni |
| author_sort | F. Vivaldi |
| collection | DOAJ |
| description | Affinity receptors have played a pivotal role in advancing bioanalysis, primarily for diagnostic and therapeutic purposes, thanks to their high selectivity toward target molecules, which enables their use in complex biofluids. While antibodies remain the gold standard, ongoing research has explored alternative receptors with improved stability, reduced size, and enhanced performance in bioanalytical and clinical applications. This works traces the evolution of affinity receptors from classical antibodies to emerging biomimetic and synthetic alternatives, including affibodies, nanobodies, aptamers, and molecularly imprinted polymers (MIPs). We highlight their molecular features, advantages, and limitations, emphasizing their use as part of the molecular toolbox for bioanalytical assay development and biosensing. Overall, the continuous diversification of affinity binders reflects the dynamic nature of this field, where the optimal receptor remains context dependent. For this reason, the research into new binders to complement or eventually replace antibodies that, for the moment, remain the choice for high-throughput applications, is still ongoing. |
| format | Article |
| id | doaj-art-7db32c32d15045d1abffc4f6f7d91e8c |
| institution | Matheson Library |
| issn | 2666-0539 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj-art-7db32c32d15045d1abffc4f6f7d91e8c2025-07-08T04:04:51ZengElsevierSensors and Actuators Reports2666-05392025-12-0110100359Exploring affinity receptors in bioanalysis: from natural binders to biomimeticsF. Vivaldi0F. Torrini1F. Spiaggia2F. Di Francesco3M. Minunni4Department of Chemistry and Industrial Chemistry, University of Pisa, 56124 Pisa, Italy; Corresponding authors.National Institute of Optics, National Research Council (INO-CNR), Via Nello Carrara 1, 50019 Sesto Fiorentino, ItalyDepartment of Pharmacy, University of Pisa, 56126 Pisa, ItalyDepartment of Chemistry and Industrial Chemistry, University of Pisa, 56124 Pisa, ItalyDepartment of Pharmacy, University of Pisa, 56126 Pisa, Italy; Corresponding authors.Affinity receptors have played a pivotal role in advancing bioanalysis, primarily for diagnostic and therapeutic purposes, thanks to their high selectivity toward target molecules, which enables their use in complex biofluids. While antibodies remain the gold standard, ongoing research has explored alternative receptors with improved stability, reduced size, and enhanced performance in bioanalytical and clinical applications. This works traces the evolution of affinity receptors from classical antibodies to emerging biomimetic and synthetic alternatives, including affibodies, nanobodies, aptamers, and molecularly imprinted polymers (MIPs). We highlight their molecular features, advantages, and limitations, emphasizing their use as part of the molecular toolbox for bioanalytical assay development and biosensing. Overall, the continuous diversification of affinity binders reflects the dynamic nature of this field, where the optimal receptor remains context dependent. For this reason, the research into new binders to complement or eventually replace antibodies that, for the moment, remain the choice for high-throughput applications, is still ongoing.http://www.sciencedirect.com/science/article/pii/S2666053925000773Affinity receptorsBioanalysisAptamersAntibodiesMolecularly imprinted polymersBiosensors |
| spellingShingle | F. Vivaldi F. Torrini F. Spiaggia F. Di Francesco M. Minunni Exploring affinity receptors in bioanalysis: from natural binders to biomimetics Sensors and Actuators Reports Affinity receptors Bioanalysis Aptamers Antibodies Molecularly imprinted polymers Biosensors |
| title | Exploring affinity receptors in bioanalysis: from natural binders to biomimetics |
| title_full | Exploring affinity receptors in bioanalysis: from natural binders to biomimetics |
| title_fullStr | Exploring affinity receptors in bioanalysis: from natural binders to biomimetics |
| title_full_unstemmed | Exploring affinity receptors in bioanalysis: from natural binders to biomimetics |
| title_short | Exploring affinity receptors in bioanalysis: from natural binders to biomimetics |
| title_sort | exploring affinity receptors in bioanalysis from natural binders to biomimetics |
| topic | Affinity receptors Bioanalysis Aptamers Antibodies Molecularly imprinted polymers Biosensors |
| url | http://www.sciencedirect.com/science/article/pii/S2666053925000773 |
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