A Comparative Study of Optical Sensing Methods for Colourimetric Bio/Chemical Detection: Cost, Scale, and Performance

This study provides a detailed comparison of three optical sensing approaches for colourimetric bio/chemical detection, focusing on cost, scalability, and performance. We examine laboratory-grade spectrophotometry, portable camera-based imaging, and low-cost LED photometry using Paired Emitter–Detec...

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Bibliographic Details
Main Authors: Cormac D. Fay, Liang Wu, Isabel M. Perez de Vargas Sansalvador
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Sensors
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Online Access:https://www.mdpi.com/1424-8220/25/13/3850
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Summary:This study provides a detailed comparison of three optical sensing approaches for colourimetric bio/chemical detection, focusing on cost, scalability, and performance. We examine laboratory-grade spectrophotometry, portable camera-based imaging, and low-cost LED photometry using Paired Emitter–Detector Diode (PEDD) charge–discharge methodology. Our findings reveal that while the LED-based PEDD system outperforms the other two methods in key sensory metrics—such as sensitivity, resolution, and limit of detection—its cost-effectiveness and scalability make it a promising solution for widespread industrial and field applications. Compared to the spectrophotometer, the LED/PEDD approach demonstrates improvements in measurement range (×16.39), dynamic range (×147.06), accuracy (×1.79), and sensitivity (×107.53). The results highlight the potential for industrial-scale adoption of LED photometry, especially for cost-effective applications in bio/chemical sensing sectors.
ISSN:1424-8220