Cultivation of <i>Chlorella</i> sp. in a Closed System Using Mining Wastewater and Simulated Flue Gas: Biomass Production and CO<sub>2</sub> Fixation Potential
<i>Chlorella</i> sp. was cultivated in a closed system using PET bottles (5 L) and with the continuous injection of air and commercial gas (98% CO<sub>2</sub>) and in simulated conditions (15% CO<sub>2</sub>, 73% N<sub>2</sub>, and 12% O<sub>2<...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Sustainable Chemistry |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-4079/6/2/11 |
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| Summary: | <i>Chlorella</i> sp. was cultivated in a closed system using PET bottles (5 L) and with the continuous injection of air and commercial gas (98% CO<sub>2</sub>) and in simulated conditions (15% CO<sub>2</sub>, 73% N<sub>2</sub>, and 12% O<sub>2</sub>). The culture medium was prepared using well water and mining wastewater, the cultivation period occurred in a 10-day cycle, and the cell growth curves were evaluated through cell counting using a Neubauer chamber. The cultivation was carried out under the following conditions: temperature at 22 °C to 25 °C; aeration rate with commercial and simulated CO<sub>2</sub> gas at 0.01 vvm; and synthetic air containing 0.042% CO<sub>2</sub>. The dry biomass productivity was 0.81 g·L<sup>−1</sup>·day<sup>−1</sup> and the maximum CO<sub>2</sub> fixation rate was 0.90 g·L<sup>−1</sup>·day<sup>−1</sup> when the microalgae were cultivated with a continuous flow of simulated waste gas and a culture medium composed of wastewater. The percentages of macromolecules obtained in the biomass cultivated in wastewater reached 20.95%, 26.48%, and 9.3% for lipids, proteins, and carbohydrates, respectively. |
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| ISSN: | 2673-4079 |