Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging
This study developed biodegradable levan–chitosan composite films for beef filet packaging, optimizing their functional properties and assessing their efficacy in food preservation. Films were prepared via solution casting using varying levan–chitosan ratios (100:0, 75:25, 50:50, 25:75, 0:100) with...
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MDPI AG
2025-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/12/2133 |
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| author | Anissa Haddar Emna Sellami Oumayma Bouazizi Assaad Sila Ali Bougatef |
| author_facet | Anissa Haddar Emna Sellami Oumayma Bouazizi Assaad Sila Ali Bougatef |
| author_sort | Anissa Haddar |
| collection | DOAJ |
| description | This study developed biodegradable levan–chitosan composite films for beef filet packaging, optimizing their functional properties and assessing their efficacy in food preservation. Films were prepared via solution casting using varying levan–chitosan ratios (100:0, 75:25, 50:50, 25:75, 0:100) with glycerol (30%) as a plasticizer. The L25:C75 formulation exhibited superior mechanical properties (tensile strength: 15.43 ± 0.04 MPa; elongation at break: 35.37 ± 1.12%) and high-water solubility (46.64 ± 0.37%). FTIR analysis confirmed intermolecular interactions between levan and chitosan. The films demonstrated strong antioxidant activity (91% ABTS•+ inhibition) and broad-spectrum antibacterial effects, with inhibition zones of 18 ± 0.30 mm against <i>E. coli</i> and 16 ± 0.20 mm against <i>S. aureus</i>. When applied to beef filets stored at 4 °C for 7 days, the L25:C75 films reduced total viable bacterial counts by 1.8 log CFU/g compared to LDPE packaging. Biodegradability tests revealed a 68% degradation rate for high-levan films (L75:C25) after 14 days in soil. These results highlight the potential of levan–chitosan films as sustainable, active packaging materials to extend food shelf life while addressing environmental concerns. |
| format | Article |
| id | doaj-art-7c7cc4e48a844e60827d032937db0a90 |
| institution | Matheson Library |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-7c7cc4e48a844e60827d032937db0a902025-06-25T13:51:15ZengMDPI AGFoods2304-81582025-06-011412213310.3390/foods14122133Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet PackagingAnissa Haddar0Emna Sellami1Oumayma Bouazizi2Assaad Sila3Ali Bougatef4Laboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax, University of Sfax, Sfax 3000, TunisiaLaboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax, University of Sfax, Sfax 3000, TunisiaLaboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax, University of Sfax, Sfax 3000, TunisiaLaboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax, University of Sfax, Sfax 3000, TunisiaLaboratory of Plants Improvement and Valorization of Agroressources, National School of Engineering of Sfax, University of Sfax, Sfax 3000, TunisiaThis study developed biodegradable levan–chitosan composite films for beef filet packaging, optimizing their functional properties and assessing their efficacy in food preservation. Films were prepared via solution casting using varying levan–chitosan ratios (100:0, 75:25, 50:50, 25:75, 0:100) with glycerol (30%) as a plasticizer. The L25:C75 formulation exhibited superior mechanical properties (tensile strength: 15.43 ± 0.04 MPa; elongation at break: 35.37 ± 1.12%) and high-water solubility (46.64 ± 0.37%). FTIR analysis confirmed intermolecular interactions between levan and chitosan. The films demonstrated strong antioxidant activity (91% ABTS•+ inhibition) and broad-spectrum antibacterial effects, with inhibition zones of 18 ± 0.30 mm against <i>E. coli</i> and 16 ± 0.20 mm against <i>S. aureus</i>. When applied to beef filets stored at 4 °C for 7 days, the L25:C75 films reduced total viable bacterial counts by 1.8 log CFU/g compared to LDPE packaging. Biodegradability tests revealed a 68% degradation rate for high-levan films (L75:C25) after 14 days in soil. These results highlight the potential of levan–chitosan films as sustainable, active packaging materials to extend food shelf life while addressing environmental concerns.https://www.mdpi.com/2304-8158/14/12/2133levanchitosanbiodegradable filmwater solubilityantibacterial activity |
| spellingShingle | Anissa Haddar Emna Sellami Oumayma Bouazizi Assaad Sila Ali Bougatef Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging Foods levan chitosan biodegradable film water solubility antibacterial activity |
| title | Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging |
| title_full | Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging |
| title_fullStr | Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging |
| title_full_unstemmed | Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging |
| title_short | Biodegradable Levan/Chitosan Composite Films: Development and Application in Beef Filet Packaging |
| title_sort | biodegradable levan chitosan composite films development and application in beef filet packaging |
| topic | levan chitosan biodegradable film water solubility antibacterial activity |
| url | https://www.mdpi.com/2304-8158/14/12/2133 |
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