Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay
Method for determining the contents of progesterone in female silkworm (Bombyx mori) moth was studied. The progesterone could be efficiently extracted out from the female silkworm moth according to following conditions: grinding the female moth powder and sifting through 80 item, using 0.1 mol·L<...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2008-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.014 |
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| _version_ | 1839607308979535872 |
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| author | GU Yan-yan HE Wei-qiang SHI Lian-gen |
| author_facet | GU Yan-yan HE Wei-qiang SHI Lian-gen |
| author_sort | GU Yan-yan |
| collection | DOAJ |
| description | Method for determining the contents of progesterone in female silkworm (Bombyx mori) moth was studied. The progesterone could be efficiently extracted out from the female silkworm moth according to following conditions: grinding the female moth powder and sifting through 80 item, using 0.1 mol·L<sup>-1</sup> HCl as dispose solvent, ether as extract solvent, and extracting for three times. Using 90 g·L<sup>-1</sup> bovine serum albumin Tris buffer as determination solvent, the contents of progesterone in female silkworm moth could be exactly and stably measured by radioimmunoassay. This method could be applied in determination of progesterone in female silkworm moth powder and its products for large numbers of samples. |
| format | Article |
| id | doaj-art-7a9dc3f1594f43f6833d7de77123956d |
| institution | Matheson Library |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-7a9dc3f1594f43f6833d7de77123956d2025-08-01T05:36:05ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-01-0134939710.3785/j.issn.1008-9209.2008.01.01410089209Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassayGU Yan-yanHE Wei-qiangSHI Lian-genMethod for determining the contents of progesterone in female silkworm (Bombyx mori) moth was studied. The progesterone could be efficiently extracted out from the female silkworm moth according to following conditions: grinding the female moth powder and sifting through 80 item, using 0.1 mol·L<sup>-1</sup> HCl as dispose solvent, ether as extract solvent, and extracting for three times. Using 90 g·L<sup>-1</sup> bovine serum albumin Tris buffer as determination solvent, the contents of progesterone in female silkworm moth could be exactly and stably measured by radioimmunoassay. This method could be applied in determination of progesterone in female silkworm moth powder and its products for large numbers of samples.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.014female silkworm mothprogesteroneradioimmunoassaycontent determination |
| spellingShingle | GU Yan-yan HE Wei-qiang SHI Lian-gen Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay 浙江大学学报. 农业与生命科学版 female silkworm moth progesterone radioimmunoassay content determination |
| title | Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay |
| title_full | Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay |
| title_fullStr | Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay |
| title_full_unstemmed | Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay |
| title_short | Determination of progesterone contents in female silkworm (Bombyx mori) moth by radioimmunoassay |
| title_sort | determination of progesterone contents in female silkworm bombyx mori moth by radioimmunoassay |
| topic | female silkworm moth progesterone radioimmunoassay content determination |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.014 |
| work_keys_str_mv | AT guyanyan determinationofprogesteronecontentsinfemalesilkwormbombyxmorimothbyradioimmunoassay AT heweiqiang determinationofprogesteronecontentsinfemalesilkwormbombyxmorimothbyradioimmunoassay AT shiliangen determinationofprogesteronecontentsinfemalesilkwormbombyxmorimothbyradioimmunoassay |