Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement
Background and Aim: Mycotoxin contamination in poultry feed, particularly with aflatoxin B1 (AFB1) and ochratoxin A (OTA), poses significant threats to broiler health, meat quality, and consumer safety. Toxin binders are commonly used to mitigate these effects; however, their impact on endogenous st...
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Veterinary World
2025-07-01
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| Series: | Veterinary World |
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| Online Access: | https://www.veterinaryworld.org/Vol.18/July-2025/6.pdf |
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| author | Erma Safitri Hery Purnobasuki Tita Damayanti Lestari Suzanita Utama Rimayanti Rimayanti Mirni Lamid Mutmainah Wardatul Jannah Siti Darodjah Goo Jang Mitsuhiro Takagi |
| author_facet | Erma Safitri Hery Purnobasuki Tita Damayanti Lestari Suzanita Utama Rimayanti Rimayanti Mirni Lamid Mutmainah Wardatul Jannah Siti Darodjah Goo Jang Mitsuhiro Takagi |
| author_sort | Erma Safitri |
| collection | DOAJ |
| description | Background and Aim: Mycotoxin contamination in poultry feed, particularly with aflatoxin B1 (AFB1) and ochratoxin A (OTA), poses significant threats to broiler health, meat quality, and consumer safety. Toxin binders are commonly used to mitigate these effects; however, their impact on endogenous stem cell activity and overall broiler performance remains underexplored. This study aimed to evaluate the efficacy of a commercial toxin binder in reducing AFB1 and OTA residues in broiler meat, inducing endogenous stem cell production, and improving growth and feed performance indices.
Materials and Methods: Twenty Cobb broilers were randomly assigned to four groups: Negative control (C−), positive control with mycotoxin-contaminated feed (C+), treatment 1 (T1: 1.1 g/kg binder), and treatment 2 (T2: 1.6 g/kg binder). Broilers were fed for 35 days. AFB1 and OTA levels in pectoral muscles were quantified using high-performance liquid chromatography, while endogenous stem cell markers (CD34+, CD45+, CD105−) in spleen tissue were assessed through flow cytometry. Growth parameters, feed conversion ratio (FCR), and performance index were also evaluated.
Results: AFB1 and OTA residues were significantly reduced in T1 and T2 compared to C+ (p < 0.05), with T2 showing the lowest levels (0.0023 μg/mL and 0.073 μg/mL, respectively). Flow cytometry revealed that T2 significantly induced endogenous stem cells (35.62% ± 2.16) compared to all other groups. The highest average daily growth occurred in T1 (68.78 ± 4.78 g/day), while the best FCR (1.38 ± 0.079) and performance index (386.2 ± 14.34) were also recorded in T1. No mortality occurred in any group.
Conclusion: Administering a toxin binder at 1.6 g/kg effectively reduced AFB1 and OTA residues and significantly activated endogenous stem cells, suggesting a protective and regenerative effect. Meanwhile, a dose of 1.1 g/kg yielded optimal growth performance and feed efficiency. These findings support the dual functional role of toxin binders in enhancing broiler meat safety and physiological resilience. |
| format | Article |
| id | doaj-art-7a4120f7f4e94f56a1cb720c76a946f5 |
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| issn | 0972-8988 2231-0916 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Veterinary World |
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| spelling | doaj-art-7a4120f7f4e94f56a1cb720c76a946f52025-07-13T12:05:41ZengVeterinary WorldVeterinary World0972-89882231-09162025-07-011871850186210.14202/vetworld.2025.1850-1862Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancementErma Safitri0https://orcid.org/0000-0002-6817-1178Hery Purnobasuki1https://orcid.org/0000-0002-0562-2058Tita Damayanti Lestari2https://orcid.org/0000-0001-8561-3452Suzanita Utama3https://orcid.org/0000-0003-3907-5969Rimayanti Rimayanti4https://orcid.org/0000-0002-6949-522XMirni Lamid5https://orcid.org/0000-0001-6539-4571Mutmainah Wardatul Jannah6https://orcid.org/0009-0001-2148-0256Siti Darodjah7https://orcid.org/0000-0003-3957-3669Goo Jang8https://orcid.org/0000-0003-3804-1871Mitsuhiro Takagi9https://orcid.org/0000-0001-9298-8287Department of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Veterinary Science, Division of Veterinary Reproduction Undergraduate Student of Veterinary Medicine Faculty, Universitas Airlangga, Surabaya, Indonesia.Department of Animal Production, Animal Husbandry Faculty, Universitas Padjadjaran, West Java Indonesia.Department of Theriogenology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.Laboratory of Theriogenology Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.Background and Aim: Mycotoxin contamination in poultry feed, particularly with aflatoxin B1 (AFB1) and ochratoxin A (OTA), poses significant threats to broiler health, meat quality, and consumer safety. Toxin binders are commonly used to mitigate these effects; however, their impact on endogenous stem cell activity and overall broiler performance remains underexplored. This study aimed to evaluate the efficacy of a commercial toxin binder in reducing AFB1 and OTA residues in broiler meat, inducing endogenous stem cell production, and improving growth and feed performance indices. Materials and Methods: Twenty Cobb broilers were randomly assigned to four groups: Negative control (C−), positive control with mycotoxin-contaminated feed (C+), treatment 1 (T1: 1.1 g/kg binder), and treatment 2 (T2: 1.6 g/kg binder). Broilers were fed for 35 days. AFB1 and OTA levels in pectoral muscles were quantified using high-performance liquid chromatography, while endogenous stem cell markers (CD34+, CD45+, CD105−) in spleen tissue were assessed through flow cytometry. Growth parameters, feed conversion ratio (FCR), and performance index were also evaluated. Results: AFB1 and OTA residues were significantly reduced in T1 and T2 compared to C+ (p < 0.05), with T2 showing the lowest levels (0.0023 μg/mL and 0.073 μg/mL, respectively). Flow cytometry revealed that T2 significantly induced endogenous stem cells (35.62% ± 2.16) compared to all other groups. The highest average daily growth occurred in T1 (68.78 ± 4.78 g/day), while the best FCR (1.38 ± 0.079) and performance index (386.2 ± 14.34) were also recorded in T1. No mortality occurred in any group. Conclusion: Administering a toxin binder at 1.6 g/kg effectively reduced AFB1 and OTA residues and significantly activated endogenous stem cells, suggesting a protective and regenerative effect. Meanwhile, a dose of 1.1 g/kg yielded optimal growth performance and feed efficiency. These findings support the dual functional role of toxin binders in enhancing broiler meat safety and physiological resilience.https://www.veterinaryworld.org/Vol.18/July-2025/6.pdfaflatoxinbroiler chickenfeed conversionhealthmycotoxin residueochratoxinperformance indexstem cellstoxin binder |
| spellingShingle | Erma Safitri Hery Purnobasuki Tita Damayanti Lestari Suzanita Utama Rimayanti Rimayanti Mirni Lamid Mutmainah Wardatul Jannah Siti Darodjah Goo Jang Mitsuhiro Takagi Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement Veterinary World aflatoxin broiler chicken feed conversion health mycotoxin residue ochratoxin performance index stem cells toxin binder |
| title | Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement |
| title_full | Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement |
| title_fullStr | Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement |
| title_full_unstemmed | Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement |
| title_short | Mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder: Implications for meat safety and performance enhancement |
| title_sort | mitigation of mycotoxin residues and activation of endogenous stem cells in broiler chickens using a toxin binder implications for meat safety and performance enhancement |
| topic | aflatoxin broiler chicken feed conversion health mycotoxin residue ochratoxin performance index stem cells toxin binder |
| url | https://www.veterinaryworld.org/Vol.18/July-2025/6.pdf |
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