Cloning of AlCyP1 gene from Alternaria longipes and its functions in adaptation to osmotic stress
In order to clarify the molecular mechanism of tobacco brown-spot disease pathogenic fungi Alternaria longipes resistance to dicarboximide fungicides (DCFs), GeneFishing technology was conducted to analyse gene differential expression among A. longipes strains with different DCFsresistant level, and...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Sprog: | engelsk |
| Udgivet: |
Zhejiang University Press
2011-03-01
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| Serier: | 浙江大学学报. 农业与生命科学版 |
| Fag: | |
| Online adgang: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.02.003 |
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| Summary: | In order to clarify the molecular mechanism of tobacco brown-spot disease pathogenic fungi Alternaria longipes resistance to dicarboximide fungicides (DCFs), GeneFishing technology was conducted to analyse gene differential expression among A. longipes strains with different DCFsresistant level, and the biological functions of the cloned AlCyP1 gene were analyzed by gene disruption. The results revealed that significant expression difference of a cyclophilin gene — AlCyP1 existed in different DCFs-resistant level strains. Using DNA walking method, the DNA sequence of AlCyP1 gene was cloned. Two translation initiation codons existed within the open reading frame of AlCyP1 gene, which coded two different length polypeptide products. The long and short polypeptide products contained 222 and 188 amino acids, respectively, in which the short one was initiated at codon 35 of the long polypeptide product. In addition, the sequence homology analysis of amino acids showed AlCyP1 sharing high homology (50.0% -61.3%) with cyclophilins of other fungi. Finally, biological functions of the AlCyP1 gene were analyzed by gene disruption, and the results showed that AlCyP1 gene involved in A. longipes osmotic stress adaptation process dependent of its expression level change. |
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| ISSN: | 1008-9209 2097-5155 |