Similar Microsatellite Allelic Distribution Between <i>Anopheles darlingi</i> Population Collected by Human Landing Catch or Mosquito Magnet Traps in French Guiana

Similar Microsatellite Allelic Distribution Between <i>Anopheles darlingi</i> Population Collected by Human Landing Catch or Mosquito Magnet Traps in French Guiana

<i>Anopheles darlingi</i> is a major malaria vector in South America. Understanding its population dynamics is critical for designing effective vector control strategies. While various <i>Anopheles</i> collection methods exist, they may sample distinct populations. Microsatel...

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Main Authors: Laetitia Ferraro, Sébastien Briolant, Mathieu Nacher, Samuel Vezenegho, Antoine Adde, Christophe Nguyen, Pascal Gaborit, Jean Issaly, Romuald Carinci, Vincent Pommier de Santi, Romain Girod, Isabelle Dusfour, Hervé Bogreau
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Tropical Medicine and Infectious Disease
Subjects:
<i>Anopheles darlingi</i>
Amazonia
population genetic structure
microsatellites
collection methods
collection time
Online Access:https://www.mdpi.com/2414-6366/10/6/174
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Summary:<i>Anopheles darlingi</i> is a major malaria vector in South America. Understanding its population dynamics is critical for designing effective vector control strategies. While various <i>Anopheles</i> collection methods exist, they may sample distinct populations. Microsatellite genotyping across nine loci was performed to characterize <i>An. darlingi</i> populations, which were collected in French Guiana between 6:30 p.m. and 7:00 a.m. using human landing catch (HLC) or Mosquito Magnet<sup>®</sup> (MM) traps. Traps were arranged in a 3 × 3 Latin square design to minimize possible effects of geographical position. Pairwise <i>F</i><sub>ST</sub> index and discriminant analyses of principal components (DAPC) were used to make comparisons. A total of 431 <i>An. darlingi</i> were analyzed. No significant genetic differentiation was observed between collection methods or time slots (<i>F</i><sub>ST</sub> values non-significant, <i>p</i> > 0.25), with DAPC revealing a single genetic cluster. Despite documented phenotypic variations, no significant population structure was detected among <i>An. darlingi</i> sampled in a rural village in French Guiana via collection methods or time slots. These findings confirm that mosquitoes collected with these various methods or time slots are suitable for the molecular studies of <i>An. darlingi</i> in French Guiana. In this context, Mosquito Magnet<sup>®</sup> traps could also represent an alternative to the now controversial human landing catch.
ISSN:2414-6366

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