Assessment of Safety and Probiotic Properties of Soil <i>Bacillus</i> from Mount Karadzica
This study evaluated the probiotic and safety potential of <i>Bacillus</i> spp. isolated from undisturbed soil in Mount Karadzica, North Macedonia. Seven isolates demonstrated strong antimicrobial activity against <i>Salmonella enterica</i> ATCC 10708, tolerance to 0.3–2% bil...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2025-02-01
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Series: | Biology and Life Sciences Forum |
Subjects: | |
Online Access: | https://www.mdpi.com/2673-9976/40/1/30 |
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Summary: | This study evaluated the probiotic and safety potential of <i>Bacillus</i> spp. isolated from undisturbed soil in Mount Karadzica, North Macedonia. Seven isolates demonstrated strong antimicrobial activity against <i>Salmonella enterica</i> ATCC 10708, tolerance to 0.3–2% bile salts, and survival at pH 3.0, indicating resilience under gastrointestinal conditions. They grew across 25–44 °C, tolerated 6.5% NaCl, and exhibited no β-hemolytic activity. Six isolates were susceptible to all antibiotics tested, except one resistant to ampicillin. The findings highlight the novelty of isolating <i>Bacillus</i> spp. from soil as potential probiotics, offering promising applications in improving dietary nutrition and functional food safety. |
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ISSN: | 2673-9976 |