Assessment of Safety and Probiotic Properties of Soil <i>Bacillus</i> from Mount Karadzica

This study evaluated the probiotic and safety potential of <i>Bacillus</i> spp. isolated from undisturbed soil in Mount Karadzica, North Macedonia. Seven isolates demonstrated strong antimicrobial activity against <i>Salmonella enterica</i> ATCC 10708, tolerance to 0.3–2% bil...

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Bibliographic Details
Main Authors: Sofija Kostandinovska, Dzoko Kungulovski, Natalija Atanasova-Pancevska
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Biology and Life Sciences Forum
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Online Access:https://www.mdpi.com/2673-9976/40/1/30
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Summary:This study evaluated the probiotic and safety potential of <i>Bacillus</i> spp. isolated from undisturbed soil in Mount Karadzica, North Macedonia. Seven isolates demonstrated strong antimicrobial activity against <i>Salmonella enterica</i> ATCC 10708, tolerance to 0.3–2% bile salts, and survival at pH 3.0, indicating resilience under gastrointestinal conditions. They grew across 25–44 °C, tolerated 6.5% NaCl, and exhibited no β-hemolytic activity. Six isolates were susceptible to all antibiotics tested, except one resistant to ampicillin. The findings highlight the novelty of isolating <i>Bacillus</i> spp. from soil as potential probiotics, offering promising applications in improving dietary nutrition and functional food safety.
ISSN:2673-9976