Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing
Aim: Acute cutaneous injuries and refractory chronic wounds represent prevalent clinical challenges in daily life. To address the impediments to wound healing, we propose a novel hydrogel-based therapeutic approach designed to prevent bacterial invasion, mitigate infection-induced persistent inflamm...
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Open Exploration Publishing Inc.
2025-05-01
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| Series: | Exploration of BioMat-X |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A101339/101339.pdf |
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| author | Shujing Li Wensheng Pan Maohu Chen Ruyue Wang Feng Chen |
| author_facet | Shujing Li Wensheng Pan Maohu Chen Ruyue Wang Feng Chen |
| author_sort | Shujing Li |
| collection | DOAJ |
| description | Aim: Acute cutaneous injuries and refractory chronic wounds represent prevalent clinical challenges in daily life. To address the impediments to wound healing, we propose a novel hydrogel-based therapeutic approach designed to prevent bacterial invasion, mitigate infection-induced persistent inflammatory responses, and reduce excessive oxidative stress, thereby enhancing the wound healing process. Methods: This study presents a method for preparing hyaluronic acid/silk fibroin (HA/SF) composite hydrogels via photo-crosslinking. HA and SF were respectively modified via methacrylation and glycidyl methacrylate to synthesize HAMA and SFMA. Mussel-inspired catechol groups were then grafted onto HAMA chain segments to prepare precursor HAC. Under photo-initiator LAP, polymerization was triggered to ultimately form a hydrogel network integrating mechanical toughness and tissue adhesiveness. Composite hydrogels with varying degrees of crosslinking are synthesized by adjusting the SFMA content. Results: The results demonstrate that this hydrogel can effectively achieve hemostasis within 20 seconds. Lap shear testing revealed that the HASF-gel-2 hydrogel exhibited the highest maximum adhesive strength of 160.3 kPa among all experimental groups. Furthermore, while the cell viability of the control group was normalized to 1, the composite hydrogel groups displayed values of 1.015, 1.085, 1.136, and 1.263, respectively, indicating favorable biocompatibility. The appropriate incorporation of SF was shown to enhance cellular proliferation. On day 3 post-wounding, the HASF hydrogel group demonstrated a wound closure rate of 41.7%, outperforming commercial products under identical conditions. Conclusions: In rat wound models, the HASF composite hydrogel significantly accelerated wound healing progression. |
| format | Article |
| id | doaj-art-74aecf55b924408087de0d25fceabdb5 |
| institution | Matheson Library |
| issn | 2996-9476 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of BioMat-X |
| spelling | doaj-art-74aecf55b924408087de0d25fceabdb52025-07-15T01:58:29ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762025-05-01221810.37349/ebmx.2025.101339Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healingShujing Li0https://orcid.org/0009-0006-2697-1536Wensheng Pan1https://orcid.org/0000-0002-2347-1695Maohu Chen2https://orcid.org/0009-0006-9627-3709Ruyue Wang3https://orcid.org/0009-0009-8163-1392Feng Chen4https://orcid.org/0000-0001-6881-6207Institute of Polymer Materials and Engineering, College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, P. R. China; Department of Gastroenterology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Institute of Gastrointestinal Diseases, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Zhejiang Provincial Engineering Laboratory of Diagnosis, Treatment and Pharmaceutical Development of Gastrointestinal Tract Tumors, Hangzhou 310014, Zhejiang, P. R. ChinaDepartment of Gastroenterology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Institute of Gastrointestinal Diseases, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Zhejiang Provincial Engineering Laboratory of Diagnosis, Treatment and Pharmaceutical Development of Gastrointestinal Tract Tumors, Hangzhou 310014, Zhejiang, P. R. ChinaInstitute of Polymer Materials and Engineering, College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, P. R. China; Department of Gastroenterology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Institute of Gastrointestinal Diseases, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Zhejiang Provincial Engineering Laboratory of Diagnosis, Treatment and Pharmaceutical Development of Gastrointestinal Tract Tumors, Hangzhou 310014, Zhejiang, P. R. ChinaInstitute of Polymer Materials and Engineering, College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, P. R. China; Department of Gastroenterology, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Institute of Gastrointestinal Diseases, Hangzhou Medical College, Hangzhou 310014, Zhejiang, P. R. China; Zhejiang Provincial Engineering Laboratory of Diagnosis, Treatment and Pharmaceutical Development of Gastrointestinal Tract Tumors, Hangzhou 310014, Zhejiang, P. R. ChinaInstitute of Polymer Materials and Engineering, College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, P. R. ChinaAim: Acute cutaneous injuries and refractory chronic wounds represent prevalent clinical challenges in daily life. To address the impediments to wound healing, we propose a novel hydrogel-based therapeutic approach designed to prevent bacterial invasion, mitigate infection-induced persistent inflammatory responses, and reduce excessive oxidative stress, thereby enhancing the wound healing process. Methods: This study presents a method for preparing hyaluronic acid/silk fibroin (HA/SF) composite hydrogels via photo-crosslinking. HA and SF were respectively modified via methacrylation and glycidyl methacrylate to synthesize HAMA and SFMA. Mussel-inspired catechol groups were then grafted onto HAMA chain segments to prepare precursor HAC. Under photo-initiator LAP, polymerization was triggered to ultimately form a hydrogel network integrating mechanical toughness and tissue adhesiveness. Composite hydrogels with varying degrees of crosslinking are synthesized by adjusting the SFMA content. Results: The results demonstrate that this hydrogel can effectively achieve hemostasis within 20 seconds. Lap shear testing revealed that the HASF-gel-2 hydrogel exhibited the highest maximum adhesive strength of 160.3 kPa among all experimental groups. Furthermore, while the cell viability of the control group was normalized to 1, the composite hydrogel groups displayed values of 1.015, 1.085, 1.136, and 1.263, respectively, indicating favorable biocompatibility. The appropriate incorporation of SF was shown to enhance cellular proliferation. On day 3 post-wounding, the HASF hydrogel group demonstrated a wound closure rate of 41.7%, outperforming commercial products under identical conditions. Conclusions: In rat wound models, the HASF composite hydrogel significantly accelerated wound healing progression.https://www.explorationpub.com/uploads/Article/A101339/101339.pdfphoto-crosslinkinghyaluronic acidsilk fibroinhemostatic repairwound dressing |
| spellingShingle | Shujing Li Wensheng Pan Maohu Chen Ruyue Wang Feng Chen Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing Exploration of BioMat-X photo-crosslinking hyaluronic acid silk fibroin hemostatic repair wound dressing |
| title | Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing |
| title_full | Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing |
| title_fullStr | Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing |
| title_full_unstemmed | Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing |
| title_short | Preparation of photo-crosslinked hyaluronic acid/silk fibroin hydrogel for promoting wound healing |
| title_sort | preparation of photo crosslinked hyaluronic acid silk fibroin hydrogel for promoting wound healing |
| topic | photo-crosslinking hyaluronic acid silk fibroin hemostatic repair wound dressing |
| url | https://www.explorationpub.com/uploads/Article/A101339/101339.pdf |
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