Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus
Canine distemper, a fatal and highly transmissible disease caused by the canine distemper virus (CDV), poses a major threat to the companion animal industry. An urgent need exists for a rapid, specific, and simple method for the detection of this disease in order to improve its prevention and contro...
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MDPI AG
2025-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/7/432 |
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| author | Zichen Zhang Zhuangli Bi Qingqing Du Miao Zhang Linying Cai Yiming Fan Jingjie Tang Mingxing Hu Shiqiang Zhu Aoxing Tang Guijun Wang Guangqing Liu Yingqi Zhu |
| author_facet | Zichen Zhang Zhuangli Bi Qingqing Du Miao Zhang Linying Cai Yiming Fan Jingjie Tang Mingxing Hu Shiqiang Zhu Aoxing Tang Guijun Wang Guangqing Liu Yingqi Zhu |
| author_sort | Zichen Zhang |
| collection | DOAJ |
| description | Canine distemper, a fatal and highly transmissible disease caused by the canine distemper virus (CDV), poses a major threat to the companion animal industry. An urgent need exists for a rapid, specific, and simple method for the detection of this disease in order to improve its prevention and control. In this research, two monoclonal antibodies (mAbs), 1D3E9 and 1H9B7, were prepared, both of which specifically recognize the nucleoprotein (N protein) of CDV, and an immunochromatographic assay for CDV detection was subsequently developed using these mAbs. The results showed that both mAbs belong to the IgG1 subclass with kappa light chains. 1D3E9 was found to recognize the linear epitope <sup>410</sup>AGPKQSQITFLH<sup>421</sup>, while 1H9B7 targeted the epitope <sup>450</sup>HFNDERFPGH<sup>459</sup>. The test strips exhibited high specificity and good stability for up to two months when stored at 4, 25, and 37 °C. The assay exhibited a sensitivity of 10<sup>2.39</sup> TCID<sub>50</sub>/0.1 mL. When compared with RT-PCR for detecting CDV in clinical samples, the concordance rate was 91.67%. Thus, this method shows great potential for facilitating rapid on-site detection of CDV and could be highly beneficial from the viewpoint of disease surveillance and control. |
| format | Article |
| id | doaj-art-727340b5e6cf4bbfb8ae6e66073f757b |
| institution | Matheson Library |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-727340b5e6cf4bbfb8ae6e66073f757b2025-07-25T13:16:45ZengMDPI AGBiosensors2079-63742025-07-0115743210.3390/bios15070432Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper VirusZichen Zhang0Zhuangli Bi1Qingqing Du2Miao Zhang3Linying Cai4Yiming Fan5Jingjie Tang6Mingxing Hu7Shiqiang Zhu8Aoxing Tang9Guijun Wang10Guangqing Liu11Yingqi Zhu12Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai 200241, ChinaCanine distemper, a fatal and highly transmissible disease caused by the canine distemper virus (CDV), poses a major threat to the companion animal industry. An urgent need exists for a rapid, specific, and simple method for the detection of this disease in order to improve its prevention and control. In this research, two monoclonal antibodies (mAbs), 1D3E9 and 1H9B7, were prepared, both of which specifically recognize the nucleoprotein (N protein) of CDV, and an immunochromatographic assay for CDV detection was subsequently developed using these mAbs. The results showed that both mAbs belong to the IgG1 subclass with kappa light chains. 1D3E9 was found to recognize the linear epitope <sup>410</sup>AGPKQSQITFLH<sup>421</sup>, while 1H9B7 targeted the epitope <sup>450</sup>HFNDERFPGH<sup>459</sup>. The test strips exhibited high specificity and good stability for up to two months when stored at 4, 25, and 37 °C. The assay exhibited a sensitivity of 10<sup>2.39</sup> TCID<sub>50</sub>/0.1 mL. When compared with RT-PCR for detecting CDV in clinical samples, the concordance rate was 91.67%. Thus, this method shows great potential for facilitating rapid on-site detection of CDV and could be highly beneficial from the viewpoint of disease surveillance and control.https://www.mdpi.com/2079-6374/15/7/432canine distemper viruscolloidal gold-based immunochromatographic stripnucleoprotein protein |
| spellingShingle | Zichen Zhang Zhuangli Bi Qingqing Du Miao Zhang Linying Cai Yiming Fan Jingjie Tang Mingxing Hu Shiqiang Zhu Aoxing Tang Guijun Wang Guangqing Liu Yingqi Zhu Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus Biosensors canine distemper virus colloidal gold-based immunochromatographic strip nucleoprotein protein |
| title | Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus |
| title_full | Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus |
| title_fullStr | Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus |
| title_full_unstemmed | Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus |
| title_short | Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus |
| title_sort | development of a colloidal gold based immunochromatographic strip targeting the nucleoprotein for rapid detection of canine distemper virus |
| topic | canine distemper virus colloidal gold-based immunochromatographic strip nucleoprotein protein |
| url | https://www.mdpi.com/2079-6374/15/7/432 |
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