Developing indica maintainer lines with intermediate amylose content by Wx functional microsatellite marker in rice
Genotypes of the 13 indica maintainer lines were detected by the Wx gene functional microsatellite marker “484/485”. The results showed that there was significant association between the Wx genotypes and amylose contents of rice. Analysis of progenies in the generations F<sub>1</sub> to...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.06.002 |
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| Summary: | Genotypes of the 13 indica maintainer lines were detected by the Wx gene functional microsatellite marker “484/485”. The results showed that there was significant association between the Wx genotypes and amylose contents of rice. Analysis of progenies in the generations F<sub>1</sub> to F<sub>3</sub> derived from “Ⅱ-32B/Yixiang 1B” confirmed that “484/485” microsatellite marker was tightly linked to and cosegregated with rice amylose content. After carrying out marker-assisted selection in F<sub>2</sub> generation, individual plants with the homozygous Wx- Ⅰ genotype selected from F<sub>3</sub> generation reached as high as 93.5%. The phenotypes such as plant type, grain shape, chalkiness, transparency, gelatinization temperature, etc. were characterized either in the field or in the lab. Finally, a total of 114 target breeding lines with Wx- Ⅰ genotype, good plant types, slender grain type, low degree of chalkiness, high degree of transparency, intermediate gelatinization temperature and amylose contents were successfully obtained from the F<sub>6</sub> generation. Ninety-two point one percent of the breeding lines had the amylose contents between 15% and 20%. Results of testcross showed that 74.5% of the breeding lines were maintainer lines. It is suggested that developing indica maintainer lines with intermediate amylose contents by Wx microsatellite marker is feasible and effective. |
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| ISSN: | 1008-9209 2097-5155 |